Expression of urinary exosomal microRNA-145-5p in diabetic kindney disease mice and the relationship with SLIT-ROBO guanosine triphosphatase activating protein 2
10.3969/j.issn.1006-6187.2025.09.012
- VernacularTitle:糖尿病肾脏疾病小鼠尿外泌体微小RNA-145-5p表达及与SLIT-ROBO鸟苷三磷酸酶激活蛋白2靶向关系的研究
- Author:
Lulu HAN
1
;
Shenghai WANG
;
Mingyan YAO
Author Information
1. 071000 保定市第一中心医院内分泌科
- Publication Type:Journal Article
- Keywords:
Diabetic kidney disease;
Urinary exosome;
MicroRNA-145-5p;
SLIT-ROBO guanosine triphosphatase activating protein 2;
Biomarker
- From:
Chinese Journal of Diabetes
2025;33(9):701-706
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of urinary exosomal microRNA-145-5p(miR-145-5p)and the targeted relationship with SLIT-ROBO guanosine triphosphatase activating protein 2(Srgap2)in diabetic kidney disease(DKD)mice.Methods A total of 11 db/db mice with random blood glucose≥16.7 mmol/L were selected to construct DKD model,and 10 C57BL/6J wild-type mice were assigned to the normal control(NC)group.The urine exosomes were isolated from each group.The expression of miR-145-5p in urinary exosomes and renal tissue were detected using RT-qPCR.Pearson correlation analysis was used to analyze the correlation between urinary exosomal miR-145-5p and UACR as well as the glycolipid metabolism indicators.Luciferase activity was performed to verify the targeting relationship between miR-145-5p and Srgap2.The expression Srgap2 and the ROCK activity in the renal tissues were detected using Western blot.Results Compared with NC group,the body weight,fasting plasma glucose(FPG),FIns,HbA1c,TC,UACR,urinary exosomal miR-145-5p and the expression of miR-145-5p in renal tissue and p-MYPT1 protein expression were significantly higher(P<0.05),while the expression of Srgap2 protein was significantly lower in DKD group(P<0.05).Pearson correlation analysis revealed that the exosomal miR-145-5p was positively correlated with UACR,FPG,HbA1c and TC(r=0.836,0.888,0.843,0.882,P<0.05).MiR-145-5p could directly target the Srgap2 protein and activate the ROCK pathway.Conclusions The expression of urinary exosomal miR-145-5p was significantly increased and the miR-145-5p/Srgap2/ROCK axis may be closely related to renal damage in DKD mice,and it is a promising diagnostic biomarker and future therapeutic target for DKD.
