Gut microbiota characteristics of children with cyclic vomiting syndrome complicated with constipation
10.3760/cma.j.issn.1673-4912.2025.04.009
- VernacularTitle:周期性呕吐综合征合并便秘患儿的肠道菌群特征
- Author:
Ningning LI
1
;
Yuan LI
;
Lin SONG
;
Yanyan DU
;
Jian WANG
;
Lina JI
;
Xiwei XU
Author Information
1. 国家儿童医学中心 首都医科大学附属北京儿童医院 100045
- Publication Type:Journal Article
- Keywords:
Gut microbiota;
Cyclic vomiting syndrome;
Constipation;
Children
- From:
Chinese Pediatric Emergency Medicine
2025;32(4):286-291
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the characteristics of gut microbiota distribution in children with cyclic vomiting syndrome（CVS）complicated by constipation.Methods:The children with CVS, aged from 1 to 16 years, who were admitted to Beijing Tsinghua Changgung Hospital from June 2022 to January 2024, were divided into constipation group and normal group(non-constipation group) according to whether they were complicated with constipation or not.The clinical data and stool samples of children were collect. The abundance, diversity and composition of intestinal flora in fecal samples of two groups were detected by metagenomics sequencing.Results:A total of 20 children with CVS were collected, including 10 patients in constipation group and 10 patients in normal group.There were no significant differences in general demographic data between the two groups, including age at admission, age at first onset, body mass index, gender distribution, disease severity, endoscopic findings, and abdominal pain patterns.Microbiome analysis yielded 470 operational taxonomic units (OTUs), with 414 OTUs identified in normal group and 56 OTUs in constipation group. The abundance and diversity of intestinal flora in constipation group were significantly lower than those in normal group. Principal coordinate analysis and principal component analysis indicated significant structural differences in gut microbiota composition between the two groups. LEfSe analysis revealed distinct taxonomic patterns between the two groups, with the normal group demonstrating predominant representation of Firmicutes at the phylum level, while the constipation group showed higher relative abundance of Bacteroidetes and Actinobacteria. KEGG pathway analysis revealed that the carbon metabolism pathways was significantly enriched in the constipation group.Conclusion:There are significant differences in intestinal flora between CVS children with and without comorbid constipation.Bacteroides and Actinomycetes play an important role in constipation of children with CVS. The diversity and metabolic function of intestinal flora may be one of the pathological mechanisms of CVS complicated with constipation.
