Senkyunolide Ⅰ alleviates LPS-induced astrocyte injury by regulating Nrf2 pathway
10.3969/j.issn.1000-484X.2025.07.021
- VernacularTitle:洋川芎内酯Ⅰ通过调控Nrf2通路缓解LPS诱导的星形胶质细胞损伤
- Author:
Haohao CAO
1
;
Tao LIU
1
;
Meixia XU
1
Author Information
1. 武汉市第四医院重症医学科,武汉 430000
- Publication Type:Journal Article
- Keywords:
Senkyunolide Ⅰ;
Sepsis-associated encephalopathy;
Astrocytes;
Nrf2
- From:
Chinese Journal of Immunology
2025;41(7):1695-1699
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effect of SenkyunolideⅠ(Sen Ⅰ)on function of astrocytes induced by lipopolysaccharide(LPS)and its mechanism.Methods:Rat neural astrocytes were induced by LPS,and the damaged cell model was constructed.Normal and injured astrocytes were treated with different concentrations of Sen Ⅰ(20,50,100,200 μmol/L),respectively.Cell proliferation was detected by CCK-8,cytotoxicity was detected,and the optimal concentration of Sen Ⅰ was determined.Astrocytes were divided into control group,LPS group,LPS+Sen Ⅰ group and LPS+Sen Ⅰ+ML385[nuclear factor E2 associated factor 2(Nrf2)inhibitor]group.Cell proliferation was detected by CCK-8 assay,expression of glial fibrillary acidic protein(GFAP)was detected by immunofluorescence assay and Western blot,mRNA and protein expression of Nrf2 was detected by qRT-PCR and Western blot,contents of TNF-α and IL-1β in supernatant of cells were detected by ELISA,and expression of glial cell line-derived neurotrophic factor(GDNF)in cells was detected by Western blot.Results:Low concentrations of Sen Ⅰ(20,50 μmol/L)were not toxic to astrocytes,while high concentra-tions(100,200 μmol/L)significantly inhibit astrocyte proliferation.The optimal concentration of Sen Ⅰ was 50 μmol/L.Compared with control group,cell proliferation ability,contents of TNF-α and IL-1β in cell supernatant,and expression of GFAP in cells were significantly increased in LPS group(P<0.01),while Nrf2 mRNA and protein level and GDNF protein level in cells were significantly decreased(P<0.01);compared with LPS group,cell proliferation ability,contents of TNF-α and IL-1β in cell supernatant,and ex-pression of GFAP in LPS+Sen Ⅰ group were significantly decreased(P<0.05),while Nrf2 mRNA and protein level and GDNF protein level in cells were significantly increased(P<0.05);compared with LPS+Sen Ⅰ group,LPS+Sen Ⅰ+ML385 group could reverse the above effects(P<0.05).Conclusion:Sen Ⅰ can inhibit the over-activation and inflammatory injury of astrocytes,and the mechanism may be related to the activation of Nrf2 pathway.
