Serum proteomics analysis of pediatric corona virus disease 2019 with encephalopathy
10.3760/cma.j.issn.1673-4912.2025.02.006
- VernacularTitle:儿童新型冠状病毒感染合并脑病的血清蛋白组学分析
- Author:
Jie ZHANG
1
;
Yanting GAO
;
Chun ZHAO
;
Yujuan WANG
;
Wei WANG
;
Yi YIN
;
Xiaowei XIN
;
Xiaoru WANG
;
Jie JIANG
;
Ruilin GAN
;
Youpeng JIN
Author Information
1. 山东第一医科大学附属省立医院小儿重症医学科，济南 250021
- Publication Type:Journal Article
- Keywords:
Corona virus disease 2019;
Children;
Encephalopathy;
Proteomics
- From:
Chinese Pediatric Emergency Medicine
2025;32(2):103-109
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the differences in protein profile expression in serum samples from children with corona virus disease 2019（COVID-19）related encephalopathy and to explore the underlying mechanisms.Methods:From December 1,2022 to January 31,2023,28 children with COVID-19 who were admitted to the Department of Pediatric Intensive Medicine at Shandong Provincial Hospital Affiliated to Shandong First Medical University were collected,including 21 patients with encephalopathy(COVID-19 with encephalopathy group) and seven patients without encephalopathy(COVID-19 without encephalopathy group).Three children from each group were selected for serum proteomic analysis using tandem mass spectrometry labeling proteomics technology.Proteins were considered significantly different if the fold change was >1.2 or <0.8，with P<0.05.Bioinformatics analysis，including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes Pathway Enrichment were performed on differentially expressed proteins.Protein-protein interaction networks were analyzed using the STRING database.Selected proteins were further validated by enzyme-linked immunosorbert assay. Results:A total of 41 differentially expressed proteins were identified between the two groups.Among these，14 proteins were upregulated and 27 proteins were downregulated in COVID-19 patients with encephalopathy compared to those without encephalopathy.Bioinformatics analysis revealed that these proteins were primarily enriched in critical signaling pathways，including complement and coagulation regulation，neutrophil degranulation and activation，and platelet degranulation.Enzyme-linked immunosorbert assay validation confirmed significant differences in key coagulation-regulating proteins(von willebrand factor upregulated，serpin family F member 2 downregulated in COVID-19 patients with encephalopatly）between the two groups.Conclusion:Coagulation dysfunction may play a role in the development of COVID-19 associated encephalopathy in children，providing valuable insights for future research.
