Regulation of miR-21 on Foxp1 and its effect on inflammation level of allergic rhinitis-asthma syndrome
10.3969/j.issn.1000-484X.2025.07.031
- VernacularTitle:miR-21对Foxp1的调控作用及其对过敏性鼻炎-哮喘综合征患者炎症水平的影响
- Author:
Nie CHEN
1
;
Jian WU
;
Zhibang HU
;
Lei XU
Author Information
1. 常州市第二人民医院暨南京医科大学第三附属医院,常州 213000
- Publication Type:Journal Article
- Keywords:
Combined allergic rhinitis and asthma syndrome;
Inflammatory factors;
miR-21;
Foxp1
- From:
Chinese Journal of Immunology
2025;41(7):1757-1762
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of miR-21 targeting Foxp1 on expressions of inflammatory factors IgE,IL-4,IL-6 and IL-13 in patients with combined allergic rhinitis and asthma syndrome(CARAS).Methods:A total of 90 subjects were collected in The Second People's Hospital of Changzhou(The Third Affiliated Hospital of Nanjing Medical University),including 45 patients with allergic rhinitis(AR)and 45 patients with CARAS.Pulmonary function instrument was used to detect pulmonary function index;RT-qPCR was used to detect expressions of miR-21 and Foxp1 in peripheral blood mononuclear cells(PBMCs);ELISA was used to detect expressions of IgE,IL-4,IL-6 and IL-13 in peripheral serum;double luciferase gene report assay was used to detect the targeting regu-latory effect of miR-21 on Foxp1;miR-21 inhibitor was transfected into PBMCs to construct miR-21 interference group cells,RT-qPCR was used to detect Foxp1 expression,ELISA was used to detect release levels of IgE,IL-4,IL-6 and IL-13 in each cell supernatant;Foxp1 overexpression lentivirus was transfected into PBMCs to construct Foxp1 overexpression cells,ELISA was used to detect levels of IgE,IL-4,IL-6 and IL-13 in supernatant of each group.Results:Compared with patients with AR,FEV1%pred and FEV1/FVC%decreased significantly in patients with CARAS;level of miR-21 in PBMCs of patients with CARAS was higher,while level of Foxp1 was lower;levels of IgE,IL-4,IL-6 and IL-13 in peripheral serum of patients with CARAS were higher;miR-21 could bind to the untranslated region of Foxp1 and negatively regulate it;the inhibition of miR-21 and the increasion of Foxp1 could reduce the release of IgE,IL-4,IL-6 and IL-13 in cell supernatant.Conclusion:miR-21 can promote the release of inflammatory factors IgE,IL-4,IL-6 and IL-13 in CARAS patients by negative regulation of Foxp1.
