Impact of magnolol on liver injury in rats with severe acute pancreatitis by regulating RIPK1/RIPK3/MLKL signaling pathway
10.3969/j.issn.1000-484X.2025.07.022
- VernacularTitle:厚朴酚调节RIPK1/RIPK3/MLKL信号通路对急性重症胰腺炎大鼠肝损伤的影响
- Author:
Guanghua CHEN
1
;
Zonglin PANG
;
Yunxin YANG
;
Hui LI
Author Information
1. 川北医学院附属医院药学部,南充 637000;川北医学院药学院,南充 637100
- Publication Type:Journal Article
- Keywords:
Magnolol;
Receptor interacting protein kinase 1/receptor interacting protein kinase 3/mixed lineage kinase do-main-like protein;
Acute pancreatitis;
Liver injury
- From:
Chinese Journal of Immunology
2025;41(7):1700-1704,1713
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the impact of magnolol(MG)on liver injury in rats with severe acute pancreatitis(SAP)through receptor interacting protein kinase(RIPK)1/RIPK3/mixed lineage kinase domain-like protein(MLKL)signaling pathway and its mechanism.Methods:The SAP rat model was induced by sodium taurocholate.The rats were randomly grouped into sham operation group(Sham group),model group(SAP group),MG group,RIPK1 inhibitor Necrostatin-1 group(Nec-1 group),and magnolol+RIPK1 inhibitor Necrostatin-1 group(MG+Nec-1 group).The pathological tissue morphology of pancreas and liver were observed by HE staining;the apoptosis rate of hepatocyte was observed by TUNEL staining;an automated biochemical analyzer was used to deter-mine serum ALT,AST and AMY contents;ELISA kits were used to detect the contents of TNF-α,IL-1β and IL-6;Western blot was used to detect the protein expression levels of p-RIPK1,RIPK1,p-RIPK3,RIPK3,p-MLKL and MLKL.Results:Compared with Sham group,pancreatic and hepatic cells in SAP group had edema and hemorrhage,and inflammatory cells increased,the expression levels of serum ALT,AST,AMY,TNF-α,IL-1β,IL-6,and p-RIPK1/RIPK1,p-RIPK3/RIPK3,p-MLKL/MLKL ratio in liver tissue were obviously increased(P<0.05).Compared with SAP group,pancreatic and liver injury in MG group and Nec-1 group were obviously reduced,the expression levels of serum ALT,AST,AMY,TNF-α,IL-1β,IL-6 and p-RIPK1/RIPK1,p-RIPK3/RIPK3,p-MLKL/MLKL ratio in liver tissue were obviously decreased(P<0.05),the MG and Nec-1 groups showed no significant differences in any measured indices(P>0.05);the effect on SAP in MG+Nec-1 group was better than that in MG group and Nec-1 group.Conclu-sion:MG can alleviate liver injury in SAP rats by inhibiting RIPK1/RIPK3/MLKL signaling pathway.
