Construction and validation of a mouse model for optically activation of oligodendrocyte precursor cells
10.16098/j.issn.0529-1356.2025.05.001
- VernacularTitle:光特异性激活少突胶质前体细胞模型小鼠的构建与验证
- Author:
Shu-yue WANG
1
;
Bei-na SHENYANG
;
Nan-xin HUANG
;
Si-wei LI
;
Bin YU
;
Yu-xin WANG
;
Lan XIAO
Author Information
1. 陆军军医大学基础医学院组织胚胎学教研室,重庆 400038
- Publication Type:Journal Article
- Keywords:
Oligodendrocyte precursor cell;
Chicken opsin 5;
G protein-coupled receptor;
Calcium imaging;
Optogenetics;
Mouse
- From:
Acta Anatomica Sinica
2025;56(5):507-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop and validate a transgenic mouse model enabling specific and inducible optogenetic activation of oligodendrocyte precursor cells(OPCs).Methods A conditional allele for the photosensitive opsin chicken opsin 5(cOpn5)(Rosa26-LSL-cOpn5)was generated using CRISPR/Cas9 technology.These mice were subsequently crossed with NG2-CreERT transgenic mice to produce NG2-CreERT;cOpn5 animals.In this model,tamoxifen administration induces Cre-mediated recombination,leading to specific expression of cOpn5 in NG2-positive OPCs.The specificity and efficiency of cOpn5 expression in OPCs were confirmed by immunofluorescent staining.Functional validation of light-induced OPC activation was performed by using calcium imaging in acute brain slices after stimulation with 470 nm blue light.Results Immunofluorescence analysis confirmed robust and specific expression of cOpn5 within NG2-positive OPCs in the brains of tamoxifen-treated NG2-CreERT;cOpn5 mice.Crucially,calcium imaging of acute brain slices from these mice demonstrated a significant increase in intracellular calcium levels in cOpn5-expressing OPCs upon stimulation with 470 nm blue light,indicating successful optogenetic activation.Conclusion We have successfully generated and validated a novel transgenic mouse model(NG2-CreERT;cOpn5)that permits specific and inducible optogenetic activation of OPCs.This model provides a novel tool for subsequent in vivo studies of the role and regulating mechanisms of OPCs in the central nervous system.
