Impacts of LncRNA XIST on proliferation,apoptosis and invasion of tongue squamous cell carcinoma cells by regulating miR-337/ADAM28
10.3969/j.issn.1001-3733.2025.05.008
- VernacularTitle:LncRNA XIST调控miR-337/ADAM28对舌鳞状细胞癌细胞增殖、凋亡、侵袭的影响
- Author:
Jiayi LI
1
;
Zhen XIAO
;
Xuelin CHEN
;
Xingyan ZHANG
;
Rong QI
Author Information
1. 161000,齐齐哈尔医学院附属第三医院口腔科
- Publication Type:Journal Article
- Keywords:
Tongue squamous cell carcinoma;
LncRNA XIST;
miR-337;
Malignant biological behavior
- From:
Journal of Practical Stomatology
2025;41(5):623-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of LncRNA XIST on proliferation,apoptosis and invasion of tongue squamous cell carcinoma(TSCC)cells by regulating miR-337/ADAM28.Methods:qRT-PCR was used to detect the expression of XIST,miR-337,ADAM28 mRNA in TSCC tissues and cells.The expression of ADAM28,CyclinD1,MMP-9,MMP-14,Caspase-3 and Bax proteins was detected by Western blot.MTT assay was applied to detect cell activity.Transwell method was applied to detect the invasion ability of cells.Cell apoptosis was detected by flow cytometry.The double luciferase reporter gene experiment was applied to verify the targeting relationship between miR-337 and LncRNA XIST,miR-337 and ADAM28.Results:Compared with the adja-cent tissues,the expression of XIST and ADAM28 mRNA in TSCC increased,the expression of miR-337 decreased(P＜0.05).Compared with human normal oral mucosa cell HOK,the expression of XIST and ADAM28 in human TSCC cell lines increased,the expression of miR-337 decreased(P＜0.05).Silencing XIST reduced the expression of CyclinD1,MMP-9 and MMP-14,up-regula-ted the expression of Caspase-3 and Bax proteins,restrained the proliferation and invasion of CAL27 cells,and induced apoptosis.Inhibition of miR-337 expression reversed the silencing XIST on the proliferation and invasion of CAL27 cells,as well as the promo-ting effect on apoptosis.The double luciferase reporter gene experiment showed that XIST targeted down-regulation of miR-337,and miR-337 targeted negative regulation of ADAM28 expression.Conclusion:LncRNA XIST down-regulates miR-337 expression,up-regulates the expression of ADAM28,induces the proliferation and invasion of TSCC cells,and inhibits cell apoptosis.
