ESM1 regulates the cell apoptosis and angiogenesis of oral squamous cell carcinoma cells through DLL4/Notch 1 pathway
10.3969/j.issn.1001-3733.2025.05.007
- VernacularTitle:ESM1通过激活DLL4/Notch1通路促进口腔鳞癌细胞的凋亡和血管生成
- Author:
Zhao LI
1
;
Xia ZHAO
;
Xiaoqing CAI
Author Information
1. 473061,南阳医学高等专科学校口腔系
- Publication Type:Journal Article
- Keywords:
Endothelial cell-specific molecule 1;
Oral squamous cell carcinoma;
Apoptosis;
Angiogenesis;
Delta-like 4/notch1
- From:
Journal of Practical Stomatology
2025;41(5):617-622
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects and underlying mechanisms of endothelial cell-specific molecule 1(ESM1)on ap-optosis and angiogenesis of oral squamous cell carcinoma(OSCC)cells.Methods:The GEO database was used to investigate the expression of ESM1 in OSCC tissue.The expression of ESM1 in OSCC cell lines of Ca127,SCC9 and Tca8113 was detected via qRT-PCR and Western blot.Tca8113 cells were grouped and transfected with various plasmid vectors.Cell apoptosis was detected using flow cytometry,while the tube formation assay was used to determine angiogenesis.Western blot was conducted to examine the levels of delta-like 4(DLL4),notch intracellular domain(NICD),hairy and enhancer of split 1(Hes1)and c-Myc.The interaction be-tween ESM1 and DLL4 was verified by co-immunoprecipitation experiments.Results:GEO database analysis showed that ESM1 was up-regulated in OSCC tissue by the datasets of GSE31056 and GSE30784,and ESM1 was also overexpressed in the OSCC cell lines.Compared with Tca8113 cells transfected with negative control siRNA,the apoptosis rate of cells transfected with siESM1 was signifi-cantly increased(11.9％±0.20％)vs(1.56％±0.20％),while the tube formation was significantly reduced.The expression of DLL4,NICD,Hes1 and c-Myc was significantly inhibited after knockdown of ESM1.Co-immunoprecipitation experiments showed that ESM1 interacted with DLL4.ESM1 knockdown and DLL4 overexpression could reverse the effects of ESM1 knockdown on apop-tosis and inhibites angiogenesis,specifically manifested by a significant increase in the expression of DLL4 and NICD,a significant decrease in cell apoptosis rate,and a significant increase in tube formation.Conclusion:ESM1 promotes the apoptosis and inhibites angiogenesis of OSCC cells by activating the DLL4/Notch1 pathway.
