Construction of a rapid and quantitative platform for loop-mediated isothermal amplification and smart-phone-based detection of Cariogenic microorganisms
10.3969/j.issn.1001-3733.2025.05.003
- VernacularTitle:基于LAMP与智能手机端对龋病致病菌快速检测平台的构建
- Author:
Jin SHANG
1
;
Xiaodong ZHANG
;
Jingfu WANG
;
Yuehui WANG
;
Chen YANG
;
Heliang WANG
;
Jiao-jiao LIU
;
Ran ZHANG
Author Information
1. 110016 沈阳,北部战区总医院口腔内科
- Publication Type:Journal Article
- Keywords:
LAMP;
Streptococcus mutans;
DNA;
quantitative analysis
- From:
Journal of Practical Stomatology
2025;41(5):595-599
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a rapid,quantitative and visualized method for the rapid detection of Streptococcus mutans(S.mutans)based on loop-mediated isothermal amplification(LAMP).Methods:Nucleic acid of S.mutans was extracted for LAMP reaction,the detection experimental conditions were optimized.Finally the positive results of the experiments were quantita-tively analyzed by using the mobile-based detection equipment.Results:The system detected the samples within 23 min and showed good specificity and sensitivity with an accuracy of 1.625×10-3 ng/μL.Positive results were captured,identified and quantified u-sing a mobile application,and the quantified results showed a good linear relationship between the absolute G value and the DNA concentration,with an equation of y=3.2x+57.133(R2=0.988 2).Conclusion:Quantitative detection of S.mutans by LAMP has the characteristics of high sensitivity,high specificity,result visualization,convenience and time-saving,and can quantify bacterial DNA.It is a new method for the early detection of caries susceptibility,and can be widely used in clinic.
