Cytotoxicity of self-adhesive resin cement under different light treatment modes
10.3969/j.issn.1001-3733.2025.05.005
- VernacularTitle:不同光照模式下自粘接树脂水门汀的细胞毒性研究
- Author:
Junchao LIU
1
;
Yidan WNAG
;
Mao YE
;
Yunbo XING
;
Hailiang YAO
;
Zhuo CHEN
;
Huili LIU
Author Information
1. 200031,上海交通大学医学院附属第九人民医院血管外科
- Publication Type:Journal Article
- Keywords:
Light mode;
Self-adhesive resin cement;
Cytotoxicity
- From:
Journal of Practical Stomatology
2025;41(5):605-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the cytotoxicity of self-adhesive resin cement RelyXTM U200 on human periodontal ligament fibro-blasts(HPDLFs)under different light treatment modes.Methods:The self-adhesive resin cement RelyXTM U200 was cured by immediate light(A),intermittent light(B),delayed light(C)and no light(D)treatment respectively.The HPDLFs were cultured with the specimen extracts under different light modes.The proliferation ability and apoptosis level of the cells in the groups were detected by CCK-8 test and flow cytometry apoptosis test.SPSS 20.0 software was used to analyze the cytotoxicity of specimens to HPDLFs.Results:At 24 h after light treatment the cytoxicity(grade)of group A,B,C and D was 1,2,3 and 4,at 72 h 1,2,2 and 3,respectively.At 72 h,the apoptosis index(%)of the cells in A,B,C and D groups was 6.38%±0.94%,16.34%±1.67%,24.13%±1.43%and 38.34%±2.75%respecitvely.Conclusion:The cytotoxicity of self-adhesive resin cement is the greatest under no light treatment mode and the least under immediate light treatment mode.