The effects of astragalus polysaccharides on bone repair in rabbit apical periodontitis
10.3969/j.issn.1001-3733.2025.05.004
- VernacularTitle:黄芪多糖对实验性兔根尖周炎骨修复的作用研究
- Author:
Linbo GAO
1
;
Zhen CAO
;
Qixin WANG
;
Long JIANG
;
Ming DONG
Author Information
1. 116044,大连医科大学口腔医学院
- Publication Type:Journal Article
- Keywords:
Apical periodontitis;
Astragalus polysaccharide;
Animal model of apical periodontitis
- From:
Journal of Practical Stomatology
2025;41(5):600-604
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of astragalus polysaccharide on the proliferation of osteoblasts and the effects of astraga-lus polysaccharide on bone repair in rabbits with apical periodontitis.Methods:CCK8 assay was used to detect of astragalus poly-saccharide on the proliferation of MC3T3-E1 cells.The expression of osteogenic marker factor BMP-2 and Runx-2 mRNA and pro-tein of MC3T3-E1 cells were detected by RT-PCR and Western blot respectively.The animal models of mandibular incisors with apical periodontitis in rabbits was established and verified by roentgenogram and HE staining.The repair ability of bone tissue was detected by roentgenogram.The expression of BMP-2 in the rabbit apical tissue was detected by immunohistochemistry.The results were compared between the treated and control rabbits(n=3).Results:Astragalus polysaccharide promoted cell proliferation after 24 h,48 h and 72 h exposure(P＜0.05).Roentgenogram and HE staining confirmed that the rabbit apical periodontitis was successfully constructed.The bone defect volume in the treated rabbits apical tissue was less and the expression of BMP-2 was high-er than that in the control rabbits(P＜0.05).Conclusion:Astragalus polysaccharide promotes the proliferation ability of osteoblasts and promotes the bone repair of rabbits with apical periodontitis.
