The effect of Sr2+ doped micro arc oxidation modified surface on osteogenic differentiation of BMSCs and osseointegration in the femurs of type 2 diabetes mellitus rats
10.3969/j.issn.1001-3733.2025.05.002
- VernacularTitle:掺锶微弧氧化钛表面对2型糖尿病大鼠骨髓基质干细胞成骨分化和股骨内种植体骨结合的影响
- Author:
Haixin QIAN
1
;
Chao QIAN
1
;
Chenyuan ZHU
1
Author Information
1. 200011,上海交通大学医学院附属第九人民医院口腔修复科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所
- Publication Type:Journal Article
- Keywords:
Sr2+;
Micro-oxidation;
Type 2 diabetic rats;
Osteogenic differentiation;
Bone generation;
Bone implant contact
- From:
Journal of Practical Stomatology
2025;41(5):588-594
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The aim of the study was to evaluate the effect of Sr2+doped micro arc oxidation treated surface on osteogen-ic differentiation of BMSCs and osseointegration in the femurs of type 2 diabetes mellitus rats.Methods:TC4 surface was treated by micro arc oxidation to create TiO2 coating,then Sr was incorporated to the coating by electrochemical treated in a strontium dichlo-ride solution.The surface topography was characterized using scanning electronic microscope.Chemical compositions were deter-mined by X-ray photoelectron spectroscopy.Type 2 diabetic rat was induced by feeding with high-fat diet and following injection of streptozotocin.BMSCs were isolated after diabetic rat was euthanized.To study the cells attachment to different samples,cells actin filaments were observed to evaluate cells attachment to different plates.MTT assay was used to evaluate the cell proliferation activity on the plates.Cells differentiation was evaluated by RT-PCR,ALP activity and immunofluorescence of osteocalcin(OCN)protein.MAO and MAO+Sr implants were inserted in the diabetic rats' femurs to evaluate the influence on new bone formation and bone im-plant contact.A software was used to compare the differences of cells proliferation and osteogenic differentiation on different plates,as well as bone regeneration and bone implant contact between the implants with different surfaces.Results:After electrochemical treatment,Sr was detected on the MAO surface.The osteogeneic-related markers were upregulated on the MAO+Sr surface.In vivo study revealed the enhanced bone generation and implant bone contact of MAO+Sr surface in diabetic rats.Conclusion:Compared to the MAO surface,Sr doped micro-arc oxidation treated surface could further improve cells osteogenic differentiation and osseointe gration in diabetics type 2 diabetic rats.
