Lipid Bilayer Fluidity Regulates CD40L-Mediated Cell Contact Interface Formation
10.16156/j.1004-7220.2025.05.029
- VernacularTitle:脂双层流动性促进CD40L介导的细胞接触面形成
- Author:
Jinhui MA
1
;
Jingjing FENG
;
Xiaoyan JIANG
;
Xiaoting DONG
;
Xiaoxi SUN
;
Jiangguo LIN
;
Ying FANG
;
Jianhua WU
Author Information
1. 华南理工大学生物科学与工程学院,生物力学研究所,广州 510006
- Publication Type:Journal Article
- Keywords:
lipid bilayer;
fluidity;
β1 integrin;
cell contact area;
two-dimensional(2D)dissociation kinetics
- From:
Journal of Medical Biomechanics
2025;40(5):1303-1308,1324
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study how lipid bilayer fluidity modulates the interaction between β1 integrin and CD40L,as well as the formation of CD40L-mediated tumor cell contact interfaces.Methods Supported lipid bilayers(SLB)with different fluidities were prepared through adjusting the 1,2-dioleoyl-sn-glycero-3-[N-(5-amino-l-carboxypentyl)iminodiacetic acid]succinyl nickel salt(DGS-NTA)content.The functionalization of lipid bilayers was achieved by anchoring fluorescently labeled CD40L molecules onto the membrane surface.The contact interface formation of PC9 cells on the functionalized lipid bilayers was observed through confocal fluorescence imaging and fluorescence recovery after photobleaching(FRAP)experiments,and data of two dimensional(2D)reaction kinetics of β1 integrin and CD40L were extracted from Zhu-Golan plots.Results The diffusion coefficient of molecules in lipid bilayer was negatively correlated with DGS-NTA content.High fluidity of lipid bilayer promoted CD40L accumulation at cell contact interface and expanded the cell contact area.The 2D dissociation constants(2D Kd)of β1 integrin-CD40L complexes were approximately 13,31 and 65 molecules/μm2 for the three lipid bilayers with high,moderate and low fluidities,respectively.Conclusions High fluidity of lipid bilayers significantly facilitates diffusion and aggregation of CD40L to the cell contact interface,thus enhancing β1 integrin-CD40L interaction and the stability of cell contact interfaces.
