Analysis of NXPH4 expression,clinical significance,and function in hepa-tocellular carcinoma based on bioinformatics methods
10.3969/j.issn.1009-9905.2025.09.003
- VernacularTitle:基于生物信息学方法分析NXPH4在肝细胞癌中的表达和临床意义及功能
- Author:
Shang-ru YANG
1
;
Wen-kai JIANG
;
Xin-ru LIU
;
Shu-ze ZHANG
;
Wen-ce ZHOU
Author Information
1. 兰州大学第二临床医学院(甘肃 兰州 730000)
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Neurexophilin 4;
Immune infiltration;
Prognosis;
Bioinformatics
- From:
Chinese Journal of Current Advances in General Surgery
2025;28(9):686-691
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of Neurexophilin 4(NXPH4)in hepatocellular carcinoma(HCC)and its clinical significance and function.Methods:Retrieved LIHC project data(mRNA expression profiles and clinical records)from TCGA,analyzed differential NXPH4 expression in HCC versus adjacent non-tumor tissues,and investi-gated correlations between NXPH4 expression and clinicopathological characteristics/prognostic outcomes in HCC.Using GOEA and GSEA to investigate potential biological functions of NXPH4 in hepatocellular carcinoma.Results:NXPH4 exhibited significant upregulation in 23 cancer types(P<0.05),with significant associations to advanced HCC progression markers including TNM stage(P<0.05),histologic grade(P<0.05),and vascular invasion(P<0.05).Clinically,elevated NXPH4 expression correlated with reduced OS(HR=1.64,95%CI:1.15-2.33,P=0.006)and DSS(HR=1.88,95%CI:1.19-2.96,P=0.007).The immune infiltration results showed that NXPH4 expression was significantly correlated with Th2 cells and Th17 cells(all P values<0.05).Furthermore,NXPH4 expression was positively correlated with the levels of several immune checkpoint markers:TIGIT(r=0.265),PD-1(r=0.297),CTLA-4(r=0.302),and LAG-3(r=0.179,all P<0.001).Gene enrichment analysis revealed NXPH4 was significantly enriched in:pattern specification process(P<0.001);receptor ligand activity(P<0.001);collagen formation(P=0.009);activation of matrix metalloproteinases(P<0.001);neuro-active ligand receptor interactions(P<0.001);and ALK2 signaling(P=0.039).Conclusion:NXPH4 is associated with clinical pathological staging and poor prognosis in HCC patients;NXPH4 is associated with oncogenic pathways and im-mune infiltration,and has high value in predicting patient prognosis and immunotherapy.
