Macrophage galactose-type lectin 1 limits mouse hematopoietic stem cell differentiation in context of inflammation by inhibiting NF-κB signaling pathway
10.3969/j.issn.1000-4718.2025.04.007
- VernacularTitle:巨噬细胞半乳糖型凝集素1通过抑制NF-κB信号通路延缓炎症诱导的小鼠造血干细胞分化
- Author:
Manchun LI
1
;
Qiang ZHAN
1
;
Mi ZOU
1
;
Ke BAI
1
;
Weiwei YI
1
;
Zhenyu JU
1
;
Zhi-yang CHEN
1
Author Information
1. 暨南大学教育部再生医学重点实验室,暨南大学发育与再生医学系,生命科学技术学院,广东 广州 510632
- Publication Type:Journal Article
- Keywords:
macrophage galactose-type lectin 1;
hematopoietic stem cells;
inflammation;
NF-κB signaling pathway
- From:
Chinese Journal of Pathophysiology
2025;41(4):679-687
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of macrophage galactose-type lectin 1(Mgl1)gene deletion on he-matopoietic stem/progenitor cells(HSPCs)under steady-state conditions and inflammation.METHODS:Mice were di-vided into a control group(wild-type)and an experimental group(Mgl1 gene-deleted).Flow cytometry was used to ana-lyze the proportions of various hematopoietic cell lineages in the peripheral blood and bone marrow of both groups,assess-ing the impact of Mgl1 gene deletion on steady-state hematopoiesis(n=3～4).Transplantation and colony-forming assays were utilized to evaluate the effects of Mgl1 gene deletion onthe repopulation capacity and colony-forming ability of HSPCs(n=5).The LPS-induced inflammation model was employed to examine the effects of Mgl1 gene deletion on the inflamma-tory response of HSPCs both in vitro and in vivo(n=5～8).Western blot and RT-qPCR were conducted to analyze the alter-ations in signaling pathways regulated by Mgl1 in the inflammatory response of HSPCs(n=3).RESULTS:(1)Mgl1 gene deletion had no significant effecton steady-state hematopoiesis(P>0.05).(2)Mgl1 gene deletion promoted inflam-mation-induced cell differentiation of HSPCs(P<0.01).(3)Mgl1 gene deletion accelerated the exhaustion of HSPCs un-der prolonged inflammatory conditions(P<0.01).(4)Mgl1 was found to regulate the inflammatory response of HSPCs via the NF-κB signaling pathway.CONCLUSION:Mgl1 gene deletion enhances the inflammatory response of HSPCs via the NF-κB signaling pathway.
