Identification and functional study of DNA damage-induced tsRNAs in HepG2 cells
10.3969/j.issn.1000-4718.2025.04.008
- VernacularTitle:DNA损伤诱导的tsRNA在HepG2细胞中的鉴定和功能研究
- Author:
Guanzi CHEN
1
;
Fuyang HONG
1
;
Yuanhao JIANG
1
;
Yuzhen ZHANG
1
;
Yusheng JIE
1
Author Information
1. 中山大学附属第三医院感染科,广东 广州 510630
- Publication Type:Journal Article
- Keywords:
DNA damage;
transfer RNA-derived small RNA;
p53 protein;
HepG2 cells
- From:
Chinese Journal of Pathophysiology
2025;41(4):688-695
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To identify the expression characteristics of transfer RNA-derived small RNAs(tsRNAs)re-sponding to DNA damage in human hepatoblastoma HepG2 cells,and to investigate their potential functions.METHODS:Based on paired HepG2 cells and TP53 gene knockout HepG2 cells,we successfully constructed a DNA damage cellular model using adriamycin(ADR).Transcriptome analysis of small noncoding RNAs was performed to systematically identi-fy a set of tsRNAs responding to ADR and involved in p53 regulation.Functional enrichment analysis was conducted using the Kyoto Encyclopedia of Genes and Genomes(KEGG).Additionally,after silencing the expression of target tsRNA genes,the biological functions of these tsRNAs in HepG2 cells were initially confirmed through CCK-8 assay and plate colo-ny formation assay.RESULTS:DNA damage induced a set of tsRNAs involved in p53 regulation,among which tRF-5-1(tRF-5_tRNA-Gly-TCC-2-1)and tRF-i-1(tRF i_tRNA-Tyr-GTA-11-1)showed the most significant up-regulation in HepG2 cells(P<0.05).Silencing of either tRF-5-1 or tRF-i-1 gene inhibited the proliferative activity of HepG2 cells(P<0.05).CONCLUSION:A group of tsRNAs responding to DNA damage can be identified in HepG2 cell model,and tsRNAs can promote the proliferative activity of HepG2 cells,suggesting that tsRNAs may play an important role in the de-velopment and progression of liver malignancies.
