A detection method of arsenic free fully automatic online digestion iodine analyzer for urinary iodine
10.3760/cma.j.cn231583-20240925-00353
- VernacularTitle:尿碘的无砷全自动在线消解碘分析仪检测方法研究
- Author:
Xiuxiu SUN
1
;
Ji'an XIE
1
;
Shudong XU
1
;
Jian JIANG
1
;
Ying WEI
1
;
Ruihan ZONG
1
;
Ningning CHEN
1
;
Hui LIU
1
Author Information
1. 安徽省疾病预防控制中心理化检验所，合肥 230601
- Publication Type:Journal Article
- Keywords:
Iodine;
Urine;
Antimony cerium catalytic spectrophotometry;
Automatic online digestion iodine analyzer
- From:
Chinese Journal of Endemiology
2025;44(11):920-924
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an arsenic free fully automatic online digestion iodine analyzer detection method for urinary iodine (hereinafter referred to as the method).Methods:Based on the principle of iodine catalyzed antimony cerium redox reaction, a fully automatic online digestion iodine analyzer was used to determine the iodine content in urine. The effectiveness of the method in terms of detection limit, precision, accuracy (determination of urinary iodine primary standard reference materials GBW09108z and GBW09110f and spiked recovery experiment), and interference experiments was validated. The method was compared with the arsenic cerium catalytic spectrophotometry method recommended by the National Reference Laboratory for Iodine Deficiency Disorders.Results:The linear range of the method was 0 - 300 μg/L, with a correlation coefficient │ r│> 0.999 5. The qualitative and quantitative detection limits were 7.41 and 18.01 μg/L, respectively. The relative standard deviation ( RSD) of urine samples with different iodine concentrations ranged from 1.0% to 1.7%. The results of the determination of iodine concentrations in urine using standard substances GBW09108z and GBW09110f were within the given standard range, with RSD < 2.5%. The range of spiked recovery rates for urine samples with different iodine concentrations was 101.3% to 104.8%, with an overall average spiked recovery rate of 103.0%. The average concentration of the baseline iodine standard solution was determined to be 116.21 μg/L, and the relative error of the concentration determination with the addition of interfering substances was less than 5.0%. The comparison results showed that there was no statistically significant difference in the measurement results between the two methods ( t = - 0.06, P = 0.952). Conclusions:The method adopts automated detection, which is simple to operate, labor-saving, and does not require the use of arsenic trioxide. It has high precision and accuracy, and is suitable for detection of large quantities of samples.
