Effect and mechanism of Macleaya cordata extract promoting the growth per-formance of Sichuan White Geese by regulating apoptosis
10.16303/j.cnki.1005-4545.2025.09.25
- VernacularTitle:博落回提取物通过调控细胞凋亡促进四川白鹅生长性能的作用机制及验证
- Author:
Yufei XIE
1
;
Pingrui YANG
;
Xifeng LI
;
Huan HUANG
;
Fuxing GUI
;
Jingxuan NI
;
Shich-eng BI
;
Yongfeng HAO
;
Chonghua ZHONG
;
Liting CAO
Author Information
1. 西南大学动物医学院,重庆 荣昌 402460
- Publication Type:Journal Article
- Keywords:
Macleaya cordata extract;
network pharmacology;
Sichuan white geese;
growth per-formance;
mechanism of action
- From:
Chinese Journal of Veterinary Science
2025;45(9):2040-2050
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect of Macleaya cordata extract(MCE)on growth performance and serum biochemistry of Sichuan White Geese,and to analyze its mechanism of action by network pharmacology and molecular docking technology combined with animal experiments verification.A total of 90 1-day-old healthy goslings were randomly divided into 5 groups with 18 goslings per group after 5 d of adaptive feeding.The control group(CON)was fed with basal diet,the antibiotic group(CTC)was supplemented with 450 mg/kg chlortetracycline premix,and the low MCE group(MCEL),medium MCE group(MCEM)and high MCE group(MCEH)were supple-mented with 200,300 and 400 mg/kg MCE,respectively.The experimental period was 21 d.On the basis of the above experiments,Network pharmacology and molecular docking technology were used to predict the core targets and signaling pathways of MCE to promote geese growth from the levels of antioxidant,immune and apoptosis,and the expression levels of the core target genes were detected by Real-time fluorescence quantitative PCR.The results showed that compared with the CON group,MCE supplementation could increase the average daily weight gain,decrease the ratio of feed to gain,significantly increase the contents of serum GH,T3,T4,TP and ALB(P＜0.05),and significantly decrease the contents of serum AST and TG(P＜0.05).Network pharmacology analysis predicted 2 active ingredient and 237 active ingredient targets,and concluded that the mechanism of MCE promoting the growth of Sichuan White Geese may be related to the role of 5 key targets such as SRC,HSP90AA1,CASP3,ESR1 and MAPK14,and the action of MAPK,apop-tosis and other signaling pathways.Molecular docking results showed that the active ingredients of sanguinarine and chelerythrine in MCE could act through MAPK14.The validation results of core targets showed that MCE could significantly reduce the mRNA expression levels of CytC,CASP2,CASP3 and CASP9 in spleen(P＜0.05)and significantly increase the mRNA expression levels of Mcl-1(P＜0.05).These results indicated that MCE could promote the growth performance of Si-chuan White Geese by regulating apoptosis,promoting the secretion of serum growth-related hor-mones and improving biochemical indicators.
