The role of BMP2/Smad8 signaling pathway in T-2 toxin-induced apoptosis of rat articular chondrocytes
10.3760/cma.j.cn231583-20240704-00192
- VernacularTitle:BMP2/Smad8信号通路在T-2毒素诱导大鼠关节软骨细胞凋亡中的作用
- Author:
Yang LI
1
;
Fuyuan LI
;
Xinhua SHAO
;
Meili YANG
;
Fuxun CHEN
;
Baihui ZHANG
;
Zhongyao ZHANG
;
Jialing CHAI
;
Ning ZOU
Author Information
1. 哈尔滨医科大学中国疾病预防控制中心地方病控制中心 国家卫生健康委员会病因流行病学重点实验室 地方病联合重点实验室（哈尔滨医科大学、贵州医科大学、西安交通大学）黑龙江省微量元素与人体健康重点实验室 黑龙江省普通高校病因流行病学重点实验室，哈尔滨 150081
- Publication Type:Journal Article
- Keywords:
T-2 toxin;
Articular cartilage;
Apoptosis;
BMP2/Smad8 signaling pathway
- From:
Chinese Journal of Endemiology
2025;44(9):689-694
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to investigate the role of bone morphogenetic protein 2 (BMP2)/Smad8 signaling pathway in T-2 toxin-induced apoptosis of rat articular chondrocytes.Methods:Primary chondrocytes from SD rats were cultured in vitro and exposed to varying concentrations of T-2 toxin (0.00, 0.32, 1.60, 8.00 ng/ml). The changes in chondrocytes survival rate were determined by CCK8, and the apoptosis changes of chondrocytes were determined by TUNEL assay kit. Using a group design, chondrocytes were cultured in complete culture media and culture media containing T-2 toxin (1.60 ng/ml), BMP2 cytokine (500 ng/ml), or T-2 toxin (1.60 ng/ml) + BMP2 cytokine (500 ng/ml), referred to as the control group, T-2 toxin group, BMP2 group, and T-2 toxin + BMP2 group, respectively. The survival rate and apoptosis changes of chondrocytes in each group were determined. The expression levels of Caspase-3, BMP2, BMP receptor Ⅱ (BMP-R Ⅱ), and Smad1/4/5/8 were determined by quantitative real-time PCR. Results:Compared with the 0.00 ng/ml of T-2 toxin group [(100.00 ± 0.00)%, (4.33 ± 0.32)%], the chondrocyte survival rates [(85.77 ± 2.96)%, (72.79 ± 2.31)%, (48.87 ± 1.83)%] of the 0.32, 1.60, and 8.00 ng/ml of T-2 toxin groups were significantly lower ( P < 0.05), and the apoptosis rates [(5.43 ± 0.32)%, (6.17 ± 0.15)%, (5.07 ± 0.13)%] were significantly higher ( P < 0.05). Compared with the control group, the T-2 toxin group had a lower survival rate and a higher apoptosis rate of chondrocytes ( P < 0.05). Compared with the T-2 toxin group, the T-2 toxin + BMP2 group had a higher survival rate and lower apoptosis rate of chondrocytes ( P < 0.05). Compared with the control group, the T-2 toxin group showed higher expression level of Caspase-3 mRNA in chondrocytes, while the expression levels of BMP2, BMP-R Ⅱ, and Smad1/4/8 mRNA were lower ( P < 0.05). Compared with the T-2 toxin group, the expression level of Caspase-3 mRNA was lower in the T-2 toxin + BMP2 group, while the expression levels of BMP2 and Smad8 mRNA were higher ( P < 0.05). Conclusion:BMP2 may partially block the apoptosis of chondrocytes caused by T-2 toxin by regulating the BMP2/Smad8 signaling pathway.
