Ginkgetin mediates the NR4A2/p53/Bax pathway to regulate autophagy and inhibit cardiomyocyte apoptosis
10.12007/j.issn.0258-4646.2025.04.002
- VernacularTitle:银杏黄酮苷介导NR4A2/p53/Bax通路调控自噬抑制心肌细胞凋亡
- Author:
Han LI
1
;
Dongsheng WEI
;
Huimin CAO
;
Xinyue WU
;
Yelei HAN
;
Zhe ZHANG
Author Information
1. 辽宁中医药大学第一临床学院,沈阳 110847;辽宁中医药大学中医脏象理论及应用教育部重点实验室,沈阳 110847
- Publication Type:Journal Article
- Keywords:
ginkgetin;
H9c2 cell;
autophagy;
apoptosis
- From:
Journal of China Medical University
2025;54(4):295-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism by which ginkgetin attenuates H9c2 cells injury.Methods H9c2 cells were divided into five groups:control,lipopolysaccharide(LPS),LPS+3-methyladenine(3-MA,an autophagy inhibitor),LPS+ginkgetin,and LPS+3-MA+ginkgetin.Cell viability and cytotoxicity were assessed using the cell CCK-8 and lactate dehydrogenase assays,respectively.Immunofluorescence staining for LC3,monodansylcadaverine staining for autophagosomes,and flow cytometry were used to measure apop-tosis rates.Quantitative real-time PCR was performed to measure the expression of NR4A2/p53/Bax pathway.Western blotting was used to detect the expression of NR4A2,p53,Bax,LC3,Beclin-1,p62,cleaved caspase-3,and Bcl-2 proteins.Results Compared to the LPS group,ginkgetin significantly increased LC3 fluorescence levels and monodansylcadaverine fluorescence intensity,decreased apoptosis,upregulated NR4A2,downregulated p53 and Bax,increased LC3,Beclin-1,and Bcl-2 proteins,and decreased p62 and cleaved caspase-3(P＜0.05).The autophagic inhibitor,3-MA,confirmed that ginkgetin protected H9c2 cells from LPS-induced apoptosis via autophagy regulation.Conclusion Ginkgetin mitigated cardiomyocyte injury by enhancing autophagic flux and alleviating LPS-induced H9c2 cells apoptosis by modulating the NR4A2/p53/Bax pathway.
