Effect of TRPV1 protein on the progression of autoimmune encephalomyelitis and the differentiation of Th1 and Th17 cells
10.12007/j.issn.0258-4646.2025.04.007
- VernacularTitle:TRPV1蛋白对自身免疫性脑脊髓炎进展及Th1和Th17细胞分化的影响
- Author:
Bufei WANG
1
;
Cong CHEN
1
;
Jianhong LI
1
Author Information
1. 海南医科大学第二附属医院神经内科,海口 570300
- Publication Type:Journal Article
- Keywords:
transient receptor potential vanilloid 1;
autoimmune encephalomyelitis;
CD4+T cells;
inflammation
- From:
Journal of China Medical University
2025;54(4):323-327,332
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of transient receptor potential vanilloid 1(TRPV1)protein on experimental autoimmune encephalomyelitis(EAE)progression and its underlying mechanism.Methods Thirty-six C57BL/6 mice were randomly divided into the control,model,and model+TRPV1 inhibitor groups,with 12 mice in each group.The EAE model was established through subcutaneous injection of myelin oligodendrocyte glycoprotein(MOG)35-55 mixed with a tuberculosis bacillus emulsion,combined with an intraperitoneal injection of pertussis toxin.Mice in the model+TRPV 1 inhibitor group were administered a daily intraperitoneal injection of TRPV1 inhibi-tor capsazepine solution at a dose of 30 mg/kg for 35 d.EAE symptoms were observed and assessed.On day 35,the mice were sacrificed,and spinal cord tissues were collected.Luxol fast blue staining was used to evaluate demyelination in the spinal cord,and flow cytometry was performed to analyze CD4+T cell differentiation in the spinal cord.Western blotting was performed to detect TRPV 1 and inflammatory response signaling protein expressions.Results Compared with the control group,mice in the model group showed increased neurological function scores and significant demyelination in the spinal cord.The flow cytometry results showed an increased proportion of CD4 T cells differentiating into Th1 and Th 17 cells in the model group.Compared with the model group,mice in the model+TRPV 1 inhibitor group exhibited decreased neurological function scores,reduced inflammatory cytokine infiltration and demyelination in the spinal cord,and a decreased proportion of CD4+T cells differentiating into Thl and Th 17 cells.TRPV 1 protein expression in the model+TRPV 1 inhibitor group was significantly inhibited,whereas p-NF-κB,caspase-1,and NLRP3 protein expressions were reduced.Conclusion TRPV 1 plays a significant role in EAE progression.TRPV 1 inhibition can inhibit the differentiation of Th1 and Th 17 cells and reduce demyelination in spinal cord tissues.
