Determination of fluoride in Mongolian milk tea powder by Taka amylase enzymolysis ion-selective electrode method
10.3760/cma.j.cn231583-20241208-00413
- VernacularTitle:Taka淀粉酶酶解离子选择电极法检测蒙古奶茶粉氟含量
- Author:
Zhixian YANG
1
;
Meina SHAN
1
;
Ke LI
1
;
Hui WANG
1
Author Information
1. 内蒙古自治区疾病预防控制中心（内蒙古自治区预防医学科学院）地方病实验室，呼和浩特 010080
- Publication Type:Journal Article
- Keywords:
Fluoride;
Ion-selective electrode;
Mongolian milk tea powder;
Taka amylase
- From:
Chinese Journal of Endemiology
2025;44(10):854-858
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an accurate, stable, and convenient Taka amylase enzymolysis ion-selective electrode method for determination of trace fluoride in Mongolian milk tea powder.Methods:The samples of instant Mongolian milk tea powder were heated and dried at 50 ℃, 1.0 g of the sample was accurately weighed and dissolved in pure water. The sample solution, Taka amylase, and total ion strength buffer solution were mixed and enzymatically hydrolyzed in a 55 ℃ water bath for 60 minutes. After cooling, filtration was carried out. The clarified liquid was collected and the fluoride content was detected by the ion-selective electrode method, and the method validation test was conducted.Results:When the standard linear concentration range was 0.2 to 5.0 mg/L, the correlation coefficient ranged from 0.999 5 to 0.999 9. The detection limit of the method was 0.006 mg/kg. The relative standard deviations ( RSD) for detecting high, medium, and low concentration samples were 0.8%, 1.9%, and 2.1%, respectively. The recovery rates ranged from 98% to 101%, 95% to 101%, and 101% to 107%, respectively. This method simultaneously measured the same batch of samples with the second method of the "National Food Safety Standard - Determination of Fluorine in Foods" (GB 5009.18-2025) - the ion-selective electrode method. The results were (23.1 ± 7.7) and (22.5 ± 7.6) mg/kg, respectively. There was no statistically significant difference between the two methods ( t = 2.01, P = 0.066). Conclusion:The Taka amylase enzymolysis ion-selective electrode method has good reproducibility and high accuracy, making it suitable for determination of trace fluoride in Mongolian milk tea powder.
