A method for determination of urinary iodine by a pure water direct dilution-ICP-MS
10.3760/cma.j.cn231583-20250117-00039
- VernacularTitle:纯水直接稀释-电感耦合等离子体质谱法测定尿碘的方法研究
- Author:
Haitao XING
1
;
Jianbin FAN
;
Dingguo JIANG
;
Shuguang HU
;
Ming CHEN
;
Wenhua CAI
;
Xiaowen LIAN
;
Zujian SU
Author Information
1. 广东省疾病预防控制中心 国家食品安全风险监测重金属参比实验室，广州 511430
- Publication Type:Journal Article
- Keywords:
Iodine;
Urine;
Pure water;
Inductively coupled plasma mass spectrometry
- From:
Chinese Journal of Endemiology
2025;44(8):679-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a pure water direct dilution-inductively coupled plasma mass spectrometry (ICP-MS) detection method for rapid determination of urinary iodine.Methods:Pure water was used to directly dilute the urine samples. The washing solution was 5.0 g/L ascorbic acid, the internal standard solution was 5.0 g/L ascorbic acid and 100 μg/L 128Te, the standard solution was prepared with the solution of lyophilized urine iodine biological component analysis reference material. The method was evaluated in terms of linear range, detection limit, quantification limit, precision and method comparision experiment. Results:The linear correlation coefficient of the standard curve for iodine concentration range from 0 to 50.0 μg/L was 0.999 7, with a detection limit of 0.2 μg/L and a quantification limit of 0.6 μg/L. The spiked recovery rates of low, medium, and high concentration iodine standard solutions added to actual urine samples were 100.8%, 99.1% and 99.7%, respectively, with relative standard deviations of 0.8%, 1.3% and 1.6%, respectively. There was no statistically significant difference ( t = - 0.14, P = 0.890) between the results of measuring actual urine and assessment urine using this method and "Determination of Iodine in Urine-Part 2: Inductively Coupled Plasma Mass Spectrometry (WS/T 107.2-2016)". Conclusions:We have successfully established a pure water direct dilution-ICP-MS method for determining urinary iodine. This method provides accurate and highly sensitive results, making it suitable for sudden public health emergencies and large-scale clinical measurement of urinary iodine.
