Identification of the first cluster infection of Brucella in Nujiang Lisu Autonomous Prefecture, Yunnan Province
10.3760/cma.j.cn231583-20241023-00374
- VernacularTitle:云南省怒江傈僳族自治州首起聚集性感染布鲁氏菌的鉴定
- Author:
Qiuju YANG
1
;
Xiangdong YANG
1
;
Peng WANG
1
;
Qinghong YUAN
1
;
Su ZHAO
1
;
Qing ZHANG
1
;
Fuping YANG
1
;
Jiao YANG
1
;
Binbin YU
1
Author Information
1. 云南省地方病防治所 云南省自然疫源性疾病防控技术重点实验室，昆明 650500
- Publication Type:Journal Article
- Keywords:
Brucella;
Bio-typing;
PCR identification;
Multilocus locus variable-number tandem repeat analysis
- From:
Chinese Journal of Endemiology
2025;44(8):622-625
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the serotype and genotype characteristics of the first cluster infection of Brucella in Nujiang Lisu Autonomous Prefecture of Yunnan Province. Methods:The information of seven suspected cluster brucellosis cases reported in Lanping County, Nujiang Lisu Autonomous Prefecture in April 2023 were collected, blood samples were collected, and the strains were isolated and cultured. Serological methods were employed for brucellosis diagnosis, bacterial species identification was performed using BCSP31-PCR and AMOS-PCR. Multilocus locus variable-number tandem repeat analysis (MLVA) was conducted for strain genotyping, followed by phylogenetic analysis comparing the strains with those from other regions of China.Results:All seven cases were diagnosed with brucellosis, they were all villagers from the same village and had daily contact with sheeps. Four suspected Brucella strains were isolated, identified as Brucella melitensis biotype by BCSP31-PCR and AMOS-PCR. The four isolated strains exhibited identical MLVA-16 genotype, with MLVA-8 genotype 42 and MLVA-11 genotype 180, belonging to the Eastern Mediterranean lineage. Compared with the MLVA-16 of the isolated strains from other regions of China, the four isolated strains formed an independent cluster. Conclusion:The first cluster infection of Brucella in Nujiang Lisu Autonomous Prefecture of Yunnan Province is caused by Brucella melitensis biotype, and the four isolated strains have showed unique MLVA genotype.
