Glioma cell-secreted Prg4 induces the expression of macrophage Dicer,a key reg-ulatory molecule for macrophage alternative activation
10.13315/j.cnki.cjcep.2025.09.003
- VernacularTitle:胶质瘤细胞分泌Prg4诱导巨噬细胞选择性活化关键调控分子Dicer表达
- Author:
Shuyi LI
1
;
Jinghan ZHONG
;
Yuqi LIU
;
Min LUO
;
Yifang PING
;
Xiuwu BIAN
Author Information
1. 陆军军医大学(第三军医大学)第一附属医院病理科,重庆 400038
- Publication Type:Journal Article
- Keywords:
glioblastoma;
macrophage;
Dicer;
Prg4
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(9):1134-1141,1148
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the key molecules mechanisms underlying the selective activation of macrophage and the regulation of Dicer expression induced by glioblastoma(GBM)cells,as well as its prognostic significance.Methods Glioblastoma conditional medium(GCM)was fractionated by molecular weight using ultrafiltration.Specif-ic molecular weight components of GCM that upregulate Dicer expression in mouse bone marrow derived macrophages(BMDMs)were identified.Secreted proteins were identified by mass spectrometry(MS).The correlation between candidate proteins and GBM prognosis was analyzed using the TCGA and CGGA database.In vitro experiments of the candidate proteins on Dicer expression in BMDMs were further carried out.Results GCM components with a molecu-lar weight of＞50 kDa significantly upregulated Dicer expression in BMDMs.MS identified five key secreted proteins:Prg4,Psap,Hexa,Aebp1,and Itih2.High expression of Prg4 was significantly positively correlated with poor progno-sis in GBM patients(P＜0.001)and was associated with the expression of selective macrophage activation markers.Recombinant Prg4 protein stimulated BMDMs and induced Dicer expression in mouse BMDMs.Conclusion This study reveals that glioma cells induce Dicer expression in macrophages by secreting Prg4,providing a theoretical basis for GBM therapeutic strategies targeting the Prg4-Dicer axis.
