Preparation of quercetin nanogel and its antibiofilm effect on Staphylococcus aureus
10.16303/j.cnki.1005-4545.2025.08.21
- VernacularTitle:槲皮素纳米凝胶的制备及对金黄色葡萄球菌的抑膜作用
- Author:
Wanhe LUO
1
;
Yongtao JIANG
;
Yonghui LU
;
Haixuan YIN
;
Shou HAN
;
Mengdi ZHANG
Author Information
1. 塔里木大学塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔 843300;塔里木大学动物科学与技术学院,新疆阿拉尔 843300
- Publication Type:Journal Article
- Keywords:
quercetin nanogel;
biofilm;
Staphylococcus aureus;
antibiofilm rate;
antibacterial rate
- From:
Chinese Journal of Veterinary Science
2025;45(8):1744-1753
- CountryChina
- Language:Chinese
-
Abstract:
Biofilm is one of the important reasons for bacterial resistance and persistent infection in Staphylococcus aureus,which poses a significant threat to the dairy farming industry in southern Xinjiang.Although quercetin has antibacterial properties,its poor hydrophilicity and low solubility limit its clinical application.Therefore,quercetin nanogel was prepared by the electrostatic interac-tion between guar gum(positive charge)and hyaluronic acid(negative charge)under the action of sodium tripolyphosphate in this study.Using encapsulation efficiency and loading capacity as indi-cators,the optimal formula was screened through single factor experiments and response surface methodology,and characterized.The minimal inhibitory concentration(MIC)and minimal mem-brane inhibitory concentration(MBIC)of quercetin nanogel against Staphylococcus aureus were determined by micro broth dilution method and micro plate semi quantitative method.The results showed that the optimal formulation of quercetin nanogel was 11.6 g/L guar gum,10.0 g/L hyalu-ronic acid and 50.0 g/L sodium tripolyphosphate.The encapsulation efficiency and loading capacity were 79.4％and 7.6％,respectively.The particle size and Zeta potential were(396.0±4.2)nm and(-25.0±0.8)mV,respectively,indicating that the nanogel had good dispersion,high stability and excellent drug loading capacity.The MIC and inhibitory rate of quercetin nanogel against Staphy-lococcus aureus were 8.0 mg/L and 83.8％,respectively.The MBIC and inhibitory rate of quercetin nanogel against Staphylococcus aureus were 8.0 mg/L and 38.3％,respectively.Therefore,the pre-pared quercetin nanogel solved the problems of low solubility and poor hydrophilicity of quercetin,improved the inhibitory effect of quercetin on the membrane of Staphylococcus aureus,and was expected to be further applied to the treatment of cow bacterial diseases in southern Xinjiang.
