Roles of prostaglandin D2 and TLR2/TLR4/NLRP3 in bone marrow-derived mac-rophages of Escherichia coli infected dairy cows
10.16303/j.cnki.1005-4545.2025.08.19
- VernacularTitle:前列腺素D2和TLR2/TLR4/NLRP3在大肠杆菌感染奶牛骨髓源巨噬细胞中的作用
- Author:
Xiaolin YANG
1
;
Pengfei GONG
;
Lili GUO
;
Jingze WU
;
Jiahui YU
;
Yinghong QIAN
;
Shuangyi ZHANG
;
Bo LIU
;
Jinshan CAO
;
Wei MAO
Author Information
1. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018;农业农村部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特 010018
- Publication Type:Journal Article
- Keywords:
E.coli;
bovine bone marrow-derived macrophages;
transcriptome;
pattern recognition re-ceptor;
prostaglandin D2
- From:
Chinese Journal of Veterinary Science
2025;45(8):1727-1734
- CountryChina
- Language:Chinese
-
Abstract:
Escherichia coli(E.coli)is a key pathogenic bacterium responsible for postpartum endo-metritis,with its colonization in the reproductive tract closely associated with endometrial damage and disruption of the ovarian cycle.This ultimately leads to infertility,causing significant economic losses to the dairy industry.Macrophages play a pivotal role in the inflammatory response.This study aims to investigate the mRNA expression profile of bovine bone marrow-derived macropha-ges following E.coli infection using RNA sequencing(RNA-seq)technology.Additionally,it seeks to identify the biological functions and signaling pathways of differentially expressed genes(DEGs)through Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses.The results demonstrated that E.coli infection induced differential expression of 4 522 genes,with 2 141 upregulated and 2 381 downregulated.These genes were primarily asso-ciated with inflammatory responses,where TLR2,TLR4,NLRP3,and PTGS2 played pivotal roles.PGD2 synthesis was mediated by TLR2,TLR4,and NLRP3.Transcriptome sequencing of bovine bone marrow-derived macrophages infected with E.coli and treated with a PGD2 inhibitor revealed a marked downregulation of TLR2 and TLR4 gene expression.qPCR validation results were highly consistent with the RNA-seq findings.This study elucidates the interactive regulatory roles of TLR2,TLR4,and NLRP3 in conjunction with PGD2,which collectively modulate bovine endome-tritis.These findings offer significant molecular insights that enhance our understanding of the pathological mechanisms underlying bovine endometritis,thereby informing its prevention and treatment strategies.
