Effects of miR-139-5p-modified UC-MSC-derived exosomes on stemness and radiotherapy sensitivity of breast cancer cells
10.12007/j.issn.0258-4646.2025.03.012
- VernacularTitle:miR-139-5p修饰UC-MSC来源外泌体对乳腺癌细胞干性和放疗敏感性的影响
- Author:
Caiyan SHI
1
;
Guoding HUANG
1
;
Chongwei LI
1
;
Feng LIU
1
Author Information
1. 海南西部中心医院肿瘤内科,海南 儋州 571700
- Publication Type:Journal Article
- Keywords:
breast cancer;
miR-139-5p;
exosome;
stemness;
radiotherapy sensitivity
- From:
Journal of China Medical University
2025;54(3):251-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of miR-139-5p-modified human umbilical cord mensenchymal stem cell(UC-MSC)-derived exosomes on stemness and radiotherapy sensitivity of breast cancer cells.Methods MCF-7 cells were divided into the control,miR-NC Exo,and miR-139-5p Exo groups.Immunofluorescence staining was used to observe the cellular uptake of exosomes.A tumor sphere formation assay determined the number of tumor spheres formed.Western blotting detected NANOG,SOX2,and OCT4 protein expression levels.MCF-7 cells were divided into control,2 Gy X ray,miR-139-5p Exo,and miR-139-5p Exo+2 Gy X ray groups.The survival rate of MCF-7 cells was detected by the MTT method.The apoptosis rate of MCF-7 cells was determined using flow cytometry.Results Compared to the control and miR-NC Exo groups,the number of tumor spheres formed by MCF-7 cells in the miR-139-5p Exo group was significantly decreased,and the relative expression levels of Nanog,SOX2,and OCT4 proteins were significantly downregulated(P＜0.05).Compared to the control group,the survival rate of MCF-7 cells in the 2 Gy X ray and miR-139-5p Exo groups was significantly decreased,and the apoptosis rate was significantly increased(P＜0.05).Compared to the 2 Gy X ray group,the survival rate of MCF-7 cells in the miR-139-5p Exo+2 Gy X ray group was significantly decreased,and the apoptosis rate was significantly increased(P＜0.05).Conclusion UC-MSC-derived exosomes modified by miR-139-5p inhibit the stemness of MCF-7 cells and enhance the radiotherapy sen-sitivity of MCF-7 cells.
