Construction of MOV10 knockout N2a cell line and its effect on Rhabdoviridae replication
10.16303/j.cnki.1005-4545.2025.08.10
- VernacularTitle:MOV10敲除N2a细胞系的构建及其对弹状病毒科病毒复制的影响
- Author:
Yongsai LIU
1
;
Yumeng SONG
1
;
Yujie BAI
1
;
Pei HUANG
1
;
Yuanyuan LI
1
;
Haili ZHANG
1
;
Hualei WANG
1
Author Information
1. 吉林大学动物医学学院/人畜共患病研究所人畜共患传染病重症诊治全国重点实验室/人畜共患病研究教育部重点实验室,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
Moloney leukemia virus 10;
CPISPR/Cas9;
knockout N2a cell lines;
Rhabdoviridae
- From:
Chinese Journal of Veterinary Science
2025;45(8):1657-1664
- CountryChina
- Language:Chinese
-
Abstract:
Moloney leukemia virus 10(MOV10)gene knockout(MKO)mouse neuro 2a(N2a)cell lines was constructed by CRISPR/Cas9(clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9)gene editing technology.First,a recombinant plasmid pMD18T-U6-sgRNA expressing MOV10 gene-specific guide RNA(sgRNA)was constructed,and then pMD18T-U6-sgRNA and pMJ920-Cas9-eGFP were co-transfected into N2a.The results showed that the MKO N2a cell line had normal cell activity and cell proliferation ability.The infection test of the MKO N2a cell line was carried out using the rabies virus(RABV)and vesicular stomatitis virus(VSV)of the Rhabdoviridae family.The results showed that the replication level of the Rhabdoviridae virus in the MKO N2a cell line was significantly enhanced.The results showed that a MKO N2a cell line was successfully constructed in this study,which provided a preliminary basis for the exploration of the biological function and antiviral mechanism of MOV10 and the develop-ment of a recombinant viral vector vaccine with RABV/VSV as the vector.
