Establishment and application of colloidal gold double-antibody sandwich assay for detection of Getah virus
10.16303/j.cnki.1005-4545.2025.08.12
- VernacularTitle:盖塔病毒胶体金双抗体夹心检测方法的建立与应用
- Author:
Jiaxin TIAN
1
;
Lijie CHANG
;
Mingzhu ZHANG
;
Jianhong LIU
;
Xiangshu QIU
;
Xinyu CAO
;
Xiuxia HE
;
Huijun LU
Author Information
1. 长春理工大学生命科学技术学院,吉林长春 130013;中国农业科学院长春兽医研究所,吉林长春 130122
- Publication Type:Journal Article
- Keywords:
Getah virus;
prokaryotic expression;
monoclonal antibody;
colloidal gold strip;
double antibody sandwich method
- From:
Chinese Journal of Veterinary Science
2025;45(8):1672-1678
- CountryChina
- Language:Chinese
-
Abstract:
Getah virus(GETV)is an arbovirus capable of infecting humans and vertebrates such as horses and pigs via blood-sucking mosquitoes,which is extremely harmful to the livestock indus-try.Current monitoring methods are time-consuming,costly,and dependent on specialized instru-ments.These characteristics hinder rapid detection in clinical samples.Therefore,the development of a simple,rapid,specific,and sensitive method for detecting GETV antigen is crucial for the pre-vention and control of GETV.In this study,a GETV E1 monoclonal antibody strain SD17/09-E1-mAb was prepared by a prokaryotic expression system for GETV E1 protein expression,and a col-loidal gold double-antibody sandwich assay encapsulating two strains of GETV E1 monoclonal an-tibody wasestablished.The results showed that the prepared colloidal gold test strips had good sen-sitivity and did not cross-react with other common porcine virus-positive tissue samples;the test strips had a high compliance rate with the IFA assay for GETV,and could be stored at 37 ℃ for one month and at room temperature for at least three months.In this study,a colloidal gold anti-surveillance test strip for rapid detection of GETV was successfully prepared,which provides a powerful tool for GETV detection.
