SMAD4 regulates the proliferation and apoptosis of ovarian granulosa cells in rats with polycystic ovary syndrome
10.12007/j.issn.0258-4646.2025.03.007
- VernacularTitle:SMAD4对多囊卵巢综合征大鼠卵巢颗粒细胞增殖和凋亡的调控作用
- Author:
Anqi DONG
1
;
Jindan ZHENG
;
Xiaomeng YU
;
Lili LIU
Author Information
1. 锦州医科大学附属第一医院妇产科,辽宁 锦州 121001
- Publication Type:Journal Article
- Keywords:
polycystic ovary syndrome;
granulosa cell;
SMAD4
- From:
Journal of China Medical University
2025;54(3):223-227
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effects of SMAD4 on the proliferation and apoptosis of ovarian granulosa cells in rats with poly-cystic ovary syndrome(PCOS).Methods A PCOS rat model was established using DHEA,and ovarian granulosa cells were extracted and cultured in vitro.The expression of SMAD4 in ovarian granulosa cells was detected by quantitative real-time PCR and Western blot-ting.SMAD4-siRNA was transfected into ovarian granulosa cells from PCOS rats.The expression of SMAD4 mRNA after transfection was determined by quantitative real-time PCR.Western blotting was performed to detect the expression levels of PCNA,BAX,and BCL-2 proteins after transfection.A CCK-8 assay was performed to evaluate cell growth after siRNA interference.Results The HE staining results revealed that the number of ovarian follicular vacuoles increased and that the number of granulosa cell layers and corpus luteum decreased,thus indicating the establishment of a PCOS model.The FSHR positivity rate exceeded 95％.SMAD4 expression in ovarian granulosa cells was higher in the PCOS group than in the control group(P＜0.05).Furthermore,siRNA effectively reduced SMAD4 expression in ovarian granulosa cells of PCOS rats(P＜0.01),promoted proliferation,and inhibited the apoptosis of granulosa cells.Con-clusion The hindered growth of ovarian granulosa cells in PCOS rats may be linked to the overexpression of SMAD4 mRNA,which sug-gests that targeting SMAD4 could be a promising approach for treating ovulatory abnormalities in patients with PCOS.
