Inhibitory effects of tirapazamine on biofilm formation of Salmonella
10.16303/j.cnki.1005-4545.2025.08.16
- VernacularTitle:替拉扎明对沙门菌生物被膜形成的抑制作用
- Author:
Heng DU
1
;
Xiwen FAN
1
;
Yaping WANG
1
;
Yao ZHAO
1
;
Chun YANG
1
;
Zhen WANG
1
Author Information
1. 北京农学院动物科学技术学院兽医学(中兽医)北京市重点实验室,北京 102206
- Publication Type:Journal Article
- Keywords:
tirapazamine;
Salmonella;
biofilm;
extracellular matrix;
anti-biofilm mechanism
- From:
Chinese Journal of Veterinary Science
2025;45(8):1705-1712
- CountryChina
- Language:Chinese
-
Abstract:
This study is to explore the antibiofilm activity of tirapazamine(TPZ)against Salmonella and the mechanism of inhibiting biofilm formation.In this study,crystal violet staining was used to determine the effect of TPZ on the minimal biofilm inhibitory concentration(MBIC)of Salmonel-la and on the development stage of Salmonella biofilm.The effects of TPZ on bacteria and biofilm of Salmonella were observed by scanning electron microscope and fluorescence microscope.Phe-nol-sulfuric acid method,spectrophotometer and BCA method were used to detect the effects of the bacteria on the content of main extracellular matrix of Salmonella.The autocoagulation and hydro-phobicity of Salmonella were measured and their effects on the cell wall of Salmonella were meas-ured by AKP method.The result showed that the MBIC value of TPZ on Salmonella ATCC 14028 was 1.563 μmol/L,it had destructive effects on both Salmonella bacteria and mature biofilm structure,it inhibited the formation of early Salmonella biofilm in a concentration-dependent manner,and inhibited the synthesis and secretion of extracellular polymers in Salmonella biofilm.The autoagglutination ability,hydrophobicity and cell wall integrity of Salmonella were affected.To sum up,the TPZ may inhibit the formation of a large number of Salmonella biofilms in the early stage of biofilm development by affecting the extracellular polymer and cell agglutination a-bility,which is expected to be a potential candidate drug against biofilm infection and provide a new idea for finding alternative antibiotics.
