Isolation,identification and genome-wide analysis of a strain of Klebsiella pneu-moniae type ST-4263 from Kole pigs
10.16303/j.cnki.1005-4545.2025.08.13
- VernacularTitle:1株柯乐猪源ST-4263型肺炎克雷伯菌的分离鉴定及全基因组分析
- Author:
Guixian ZHOU
1
;
Shihui WU
1
;
Minle WANG
1
;
Yixiao LIAO
1
;
Shuang LI
1
;
Zemin YANG
1
;
Ming WEN
1
;
Simei XIAN
1
;
Ying YANG
1
Author Information
1. 贵州大学动物科学学院,贵州贵阳 550025;贵州省动物生物制品工程技术研究中心,贵州贵阳 550025
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
whole gene sequencing;
virulence factor;
drug resistance gene
- From:
Chinese Journal of Veterinary Science
2025;45(8):1679-1687,1695
- CountryChina
- Language:Chinese
-
Abstract:
The 16S rRNA sequencing,whole genome sequencing and drug sensitivity tests were used to identify the isolates molecularly and to detect and analyse their virulence genes,resistance genes and drug resistance.The results showed that the isolate was highly homologous to Klebsiella pneumoniae X4 and located on the same branch by 16S rRNA sequence analysis,and it was named as KLKp10.Whole genome sequencing results showed that the KLKp10 genome was 5 342 841 bp in length,containing 5 138 genes,346 repetitive segments,6 rRNAs and 81 tRNAs,with a GC con-tent of 57.30％.MLST analysis showed that KLKp10 belongs to the ST-4263 type.The functions of 4 097 of the genes encoding proteins were classified and annotated by COG,and there were also 382 genes with unknown functions.A total of 50 functional classifications were involved in the an-notation results based on the GO database;33 kinds of signaling pathways were covered based on the signaling pathway annotations in the KEGG database.A total of 443 virulence genes were screened in the VFDB database,of which 339 belonged to the Set A database and could encode 124 virulence factors.The 101 resistance genes were predicted by comparing with the CARD database,among which there were more resistance genes against β-lactam antibiotics.The results of drug sensitivity test showed that KLKp10 was highly sensitive to ceftazidime,gentamicin,azithro-mycin,chloramphenicol,norfloxacin,ofloxacin,and enrofloxacin;moderately sensitive to ceftriax-one,neomycin,kanamycin,and streptomycin;and resistant to ciprofloxacin,tetracycline,amoxicil-lin,and penicillin.In this study,we systematically revealed the gene-wide characterization,virulence factors and drug resistance of Klebsiella pneumoniae KLKp10 of Kole pig origin,which provides important data support for the study of Klebsiella pneumoniae at the overall level of its genome.
