Expression of ELAVL family in glioma and their clinicopathological significance
10.13315/j.cnki.cjcep.2025.09.005
- VernacularTitle:胶质瘤中ELAVL家族的表达及临床意义
- Author:
Qingqing YANG
1
;
Wenjun LUO
1
;
Cuiyun SUN
1
;
Shizhu YU
1
;
Xuexia ZHOU
1
Author Information
1. 天津医科大学总医院/天津市神经病学研究所神经病理研究室/天津市神经损伤变异与再生重点实验室/教育部中枢创伤修复与再生重点实验室,天津 300052
- Publication Type:Journal Article
- Keywords:
gliomas;
ELAVL family;
prognosis;
proliferation
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(9):1149-1155
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression and clinical significance of the embryonic lethal abnormal vi-sion-like(ELAVL)family in glioma.Methods Pan-cancer and glioma-specific analyses of mRNA expression profiles of the ELAVL family were analyzed using the TCGA and GTEx databases.The association between ELAVL family ex-pression and survival of glioma patients was evaluated via the gene expression profiling interactive analysis 2(GEPIA2)database.The expression level of ELAVL2 protein in human glioma tissues and non-tumor control brain tissues was ver-ified by immunohistochemistry,and the relationship between its expression and prognosis was analyzed based on the fol-low-up data of patients.Western blot was performed to assess ELAVL2 protein levels in human immortalized astrocytes of UC2 and seven glioma cell lines.Overexpression of ELAVL2 in glioma cells was achieved to evaluate its impact on cell proliferation using in vitro assays.Results Compared to normal tissues,the ELAVL family exhibited distinct ex-pression patterns across various cancers.In glioma,ELAVL1 was significantly upregulated,while ELAVL2,ELAVL3 and ELAVL4 were markedly downregulated.Survival analysis revealed that low ELAVL2 expression was an independent prognostic factor for poor survival in glioma patients(P＜0.05).Immunohistochemistry confirmed that the expression of ELAVL2 decreased with the increase of glioma grade,and its low expression indicated a poor prognosis for patients(P＜0.001).Overexpression of ELAVL2 inhibited glioma cell proliferation in vitro(P＜0.001),suggesting its tumor-suppressive role.Conclusion The ELAVL family members play a critical role in glioma progression.ELAVL2 downregulation serves as a marker for adverse clinical outcomes and represents a potential therapeutic target for glioma therapy.
