Construction of a multi-epitope gene vaccine for chicken infectious bronchitis and evaluation of its immunization effect
10.16303/j.cnki.1005-4545.2025.09.06
- VernacularTitle:鸡传染性支气管炎多表位基因疫苗的构建及其免疫效果评价
- Author:
Tingting LIU
1
;
Xinyuan WANG
;
Xiaochen ZHU
;
Haoyu LIU
;
Dongchao ZHANG
;
Tian-ming JIN
Author Information
1. 天津农学院动物科学与动物医学学院,天津市农业动物繁育与健康养殖重点实验室,天津 300392
- Publication Type:Journal Article
- Keywords:
chicken infectious bronchitis;
multi-epitope vaccine;
S1 protein;
recombinant plasmid
- From:
Chinese Journal of Veterinary Science
2025;45(9):1857-1867
- CountryChina
- Language:Chinese
-
Abstract:
To construct a safe and effective multi-epitope vaccine against the S1 protein of chicken infectious bronchitis virus(IBV).In this study,homologous and non-homologous dominant epitopes of IBV M41,T,QX and H120 virulent strain S1 proteins were screened by various online bioprediction software,respectively,and a new peptide W with high immunogenicity was construc-ted by connecting the screened B-cell and T-cell epitopes with a linker peptide.W was ligated to the truncated sequence of the four viral strains by T2A yietding to the eukaryotic expression vector pEGFP-N1,and it was identified by PCR and double digestion,the obtained recombinant plasmid was transfected into HEK293A cells and target protein expression was measured by Western blot.The constructed plasmid was injected intramuscularly twice to detect the antibody level,cytokine level,and peripheral blood T cell subsets were detected after two immunizations.The epitope pro-tein W was successfully constructed,which was structurally stable,antigenic,and soluble;the re-combinant plasmid pEGFP-WMQtH,pEGFP-W,and pEGFP-MQtH matched the expected size;anti-IBV IgG antibody levels in pEGFP-N1 was increased greatly compared to the PBS group.cyto-kines IL-2,and γ interferon(IFN-γ)were increased greatly(P＜0.05);peripheral blood CD4+/CD8a value(P＜0.05)was increased greatly.The W epitope protein was successfully constructed,which can effectively activate the humoral immunity and cellular immunity against four infectious bronchitis viruses(IBV),laying a foundation for the development of an effective vaccine against IB.
