Anthocyanins alleviate pentatetrazene-induced epileptic seizures in rats by inhibiting cuproptosis
10.16557/j.cnki.1000-7547.2025.05.011
- VernacularTitle:花青素通过抑制铜死亡缓解戊四氮诱导的大鼠痫性发作
- Author:
Rui NING
1
;
Xiangwei ZHANG
;
Shuhua WU
;
Zhongbo HU
;
Jiangtao PENG
;
Ke GUO
;
Jianmin LI
Author Information
1. 滨州医学院附属医院神经外科,滨州 256603
- Publication Type:Journal Article
- Keywords:
epilepsy;
cuproptosis;
cuprous ion;
ferredoxin1(FDX1);
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(5):625-634
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of anthocyanins(C3G)on cuproptosis in chronic epileptic rats.Methods:Chronic epileptic rat model was induced by pentatetrazol(PTZ),and 90 SD rats were randomly divided into control group,PTZ group,elesclomol(ELC)group,tetrathiomolybdate(TTM)group,C3G group and ELC+C3G group.The grade,latency and frequency of seizures were recorded in each group.electroencephalogram(EEG)was used to detect abnormal electrical discharge in the brain.The action potential of hippocampal neurons was measured by patch-clamp technique.The contents of glutathione(GSH)and cuprous ions(Cu+)in hippocampus were determined by kit.Neuron damage in hippocampus was evaluated by Nissl staining.The expression of ferredoxin1(FDX1)and lipoic acid synthase(LIAS)in hippocampus was analyzed by immunohistochemistry and Western blot.Results:Compared with the control group,the rats in the PTZ group exhibited epileptic-like seizures,suggesting that the modeling was successful.Com-pared with other epileptic groups,the ELC group showed increased seizure grade,more abnormal discharges,shortened latency period,enhanced neuronal excitability,decreased Nissl particles,elevated Cu+levels,decreased GSH levels,and increased expressions of FDX1 and LIAS.The reverse was observed in C3G group(P＜0.05).Neuron damage in ELC+C3G group was less severe than that in ELC group,but more than that in PTZ group(P＜0.05).Neuron dam-age in TTM group was less severe than that in PTZ group,but more severe than that in C3G group(P＜0.05).Conclusion:cuproptosis exists in hippocampus of rats with chronic epilepsy,and the C3G can significantly inhibit cu-proptosis and alleviate the occurrence and development of chronic epilepsy.
