Transcriptome analysis of differentially expressed genes in the mice sciatic nerve in the early stage of Wallerian degeneration in vitro
10.16557/j.cnki.1000-7547.2025.05.012
- VernacularTitle:体外培养小鼠坐骨神经在沃勒变性早期的差异表达基因转录组分析
- Author:
Danyang XU
1
;
Qi ZHANG
1
;
Yanyi LI
1
;
Yunjing DU
1
;
Jinkun WEN
1
Author Information
1. 广东药科大学基础医学院,广州 510006
- Publication Type:Journal Article
- Keywords:
peripheral nerve injury(PNI);
Wallerian degeneration(WD);
transcriptome analysis;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(5):635-642
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the differentially expressed genes and related functional pathways of mouse sciatic nerves of Schwann cells(SCs)in early in vitro Wallerian degeneration(WD).Methods:The sciatic nerves of adult male C57BL/6J mice were Wallerian degeneration in vitro,and total RNA was extracted and transcriptome sequencing was performed at 3 h and 6 h after degeneration,respectively.The differentially expressed genes(DEGs),gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment were analyzed by bioinformatics.Results:Compared with the Control group,3961 and 5538 DEGs were screened in the WD 3 h group(WD3h)and the 6 h group(WD6h)of in vitro,respectively.The most significantly up-regulated genes mainly included molecules related to inflammation and immunity and neurotrophic factors.GO analysis showed that DEGs in both groups were enriched in positive transcriptional regulation and metabolic processes.KEGG pathway enrichment analysis revealed that DEGs were mainly concentrated in TNF signaling pathway,MAPK signaling pathway and ribosome production.Conclusion:At the early stage of WD,SCs up-regulates the genes related to inflammation and immunity to promote the progression of WD and secrete neurotrophic factors to support the survival of neurons,accompanied by the activation of TNF signaling path-way and MAPK signaling pathway.
