Isoorientin inhibits development of oral squamous cell carcinoma through cGAS-STING signaling pathway mediated immune response
10.3969/j.issn.1000-484X.2025.04.010
- VernacularTitle:异荭草素通过cGAS-STING信号通路介导的免疫反应抑制口腔鳞状细胞癌发展
- Author:
Bing WAN
1
;
Shan ZHANG
;
Wenchao YANG
;
Xu YANG
;
Xinbo WANG
;
Yuan LIANG
Author Information
1. 漯河医学高等专科学校口腔系,漯河 462000
- Publication Type:Journal Article
- Keywords:
Isoorientin;
Oral squamous cell carcinoma;
cGAS-STING signaling pathway;
Immune response
- From:
Chinese Journal of Immunology
2025;41(4):828-833
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Isoorientin(Iso)on development of oral squamous cell carcinoma(OSCC)and the regulation on immune response mediated by cGAS-STING signaling pathway.Methods:CAL 27 cells were divided into control group,low,medium and high concentration Iso groups and high concentration Iso+cGAS inhibitor group(high concentration Iso+RU.521 group).Proliferation activity of CAL 27 cells was detected by MTT;TUNEL method was applied to detect apoptosis of CAL 27 cells;Transwell chamber test was applied to detect migration and invasion of CAL 27 cells;Western blot was applied to detect expressions of cGAS-STING pathway related proteins in CAL 27 cells;the tumor transplantation model of mice was prepared,and the tumor inhibi-tion rate was calculated.At the same time,peripheral blood was taken to detect the differentiation of T lymphocyte subsets in peripheral blood of mice in each group by flow cytometry.Results:Compared with control group,proliferation activity,numbers of migration and invasive cells in Iso treated groups were decreased,apoptosis rate,expressions of cGAS,STING protein,and phosphorylation expres-sions of TBK1,IRF3,tumor inhibition rate of mice,and percentages of CD4+T,CD8+T cells in peripheral blood were increased(P<0.05);further use of cGAS inhibitors reversed the inhibitory effect of Iso on development of OSCC and the promotion on cGAS-STING signaling pathway(P<0.05).Conclusion:Iso can inhibit the development of OSCC by activating the immune response mediated by cGAS-STING signaling pathway.
