The electrophysiological characteristics of striatal medium spiny neurons expressing D1 and D2 receptors in mice
10.16557/j.cnki.1000-7547.2025.05.007
- VernacularTitle:小鼠纹状体D1和D2中型多棘神经元的电生理特性研究
- Author:
Xiaoli LIANG
1
;
Bingyue WANG
;
Aina FENG
;
Bin ZHANG
;
Xianqi WANG
Author Information
1. 空军军医大学第一附属医院急诊科,西安 710032
- Publication Type:Journal Article
- Keywords:
striatum;
D1/D2 medium spiny neurons(D1/D2-MSNs);
spontaneous firing;
dopamine(DA);
action potential(AP);
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(5):599-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the electrophysiological characteristics of medium spiny neurons(MSNs)expressing D1 or D2 dopamine receptors in the striatum of mice,providing a theoretical basis for exploring their roles in motor disorders,reward,and anxiety-depression.Methods:Six male adult D1-tdTomato and D2-tdTomato mice were used.After anesthesia and decapitation,the brains were quickly removed,and coronal slices were prepared.Whole-cell patch recording were employed to record spontaneous firing,resting membrane potential,and action potential parameters of D1 and D2 MSNs in the striatum.Comparative analyses were conducted to identify differences between the two types of neurons.Results:The frequency of spontaneous excitatory postsynaptic currents(sEPSCs)in D1-MSNs was signifi-cantly lower than that in D2-MSNs(P＜0.001),but there was no difference in amplitude;whereas no significant differences were observed in the frequency or amplitude of spontaneous inhibitory postsynaptic currents(sIPSCs)between D1-MSNs and D2-MSNs.Compared with D1-MSNs,D2-MSNs had a lower resting membrane potential(P＜0.01),a significantly lower rheobase for action potential generation(P＜0.01),and the frequency of action potentials induced by depolarizing current injection was significantly higher in D2-MSNs(P＜0.001).Following perfusion with dopamine(60 μmol/L),the frequency of action potentials in D1-MSNs significantly increased(P＜0.001),while the frequency of action potentials in D2-MSNs significantly decreased(P＜0.001).Conclusion:There are significant differences in the electrophysiological properties between D1-MSNs and D2-MSNs in the striatum of mice.
