The projections from the central amygdala to the dorsal raphe nucleus mediates the spontaneous activity of mice
10.16557/j.cnki.1000-7547.2025.05.010
- VernacularTitle:杏仁中央核对中缝背核的神经投射介导小鼠的自主活动
- Author:
Chi GENG
1
;
Bin HU
;
Xiaojie WANG
Author Information
1. 徐州医科大学基础医学院生物化学教研室,徐州 221000;徐州医科大学江苏省脑病生物信息重点实验室,徐州 221000;徐州医科大学基础医学国家级实验教学示范中心,徐州 221000
- Publication Type:Journal Article
- Keywords:
central amygdala(CeA);
dorsal raphe nucleus(DRN);
serotonergic neuron;
inward current;
locomo-tor activity;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(5):619-624
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to investigate the effects on the behavior of mice by optogenetic activation the neural projections from the central amygdala(CeA)to the dorsal raphe nucleus(DRN).Methods:AAV-hSyn-eYFP virus was injected into CeA of C57BL/6J mice,and the projections from CeA to DRN were observed by immunofluores-cent staining.The AAV-hSyn-ChR2-mCherry virus was injected into CeA,and AAV-DIO-eYFP was injected into DRN in Ser-Cre mouse to specifically label the serotonergic neurons.Patch-clamp recording combined with optogenetic tech-nology were employed to record the light-evoked inward currents of DRN serotonergic neurons.AAV-hSyn-ChR2-mCher-ry virus was injected into CeA and optical fiber was implanted into DRN,and the effects of optogenetic activation of the CeA-DRN neural circuit on the locomotor ability of mice was studied by open field test.Results:Four weeks after injec-tion of AAV-hSyn-eYFP virus into CeA of C57BL/6J mice,axon terminals from CeA eYFP-cells were observed in the DRN.Activation of the neural projections from the CeA to the DRN induced inhibitory inward currents in DRN seroton-ergic neurons.Activation of the projections from CeA to DRN circuit decreased the total distance and the time of the center of the open field in mice,and increased the immobility time of mice.Conclusion:The CeA neurons directly pro-ject to the DRN.Activation of CeA-DRN circuit exerts an inhibitory regulatory effect on DRN serotonergic neurons and reduces the locomotor ability of mice.
