Catalpol regulates expression of lnc-NONHSAT071210 in salivary gland epithelial cells for therapy mechanism of Sj?gren's syndrome model mice
10.3969/j.issn.1000-484X.2025.04.005
- VernacularTitle:梓醇调控涎腺上皮细胞lnc-NONHSAT071210表达治疗干燥综合征模型小鼠的作用机制
- Author:
Weiqian HE
1
;
Chunfeng WU
;
Xiaofen LI
;
Yuan LIU
;
Yujie GUO
Author Information
1. 柳州市人民医院风湿免疫科,柳州 545200
- Publication Type:Journal Article
- Keywords:
NOD mice;
Catalpol;
Sj?gren's syndrome;
Salivary gland epithelial cells;
Long non-coding RNA
- From:
Chinese Journal of Immunology
2025;41(4):798-802
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe therapeutic effect of catalpol on Sj?gren's syndrome(SS)model mice and explore further mechanism through vitro experiments.Methods:Eight-week-old NOD mice were given catalpol 100 mg/kg by gavage for 8 weeks.Serum levels of IFN-γ and IL-17 were measured.Pathological of salivary glands were observed.lnc-NONHSAT071210 shRNA trans-fected salivary gland epithelial cell lines were treated with 50 μmol/L catalpol,and expression of lnc-NONHSAT071210 was detected.Cells were intervented by 10 ng/ml IFN-γ,lnc-NONHSAT071210 shRNA and catalpol treatment for 72 h,cell proliferation and expressions of IL-17 and IFN-γ were detected.Results:Compared with control group,expressions of IFN-γ and IL-17 were decreased(P<0.05),pathology of salivary gland showed that infiltration of lymphocytes was reduced and destruction of gland structure was significantly reduced in catalpol group.Compared with IFN-γ intervention group,catalpol treatment significantly increased prolifera-tion of salivary gland epithelial cells,and decreased expressions of IFN-γ and IL-17(P<0.05).After catalpol treatment,expression of lnc-NONHSAT071210 was decreased(P<0.05).Moreover,IFN-γ and IL-17 expressions were decreased by catalpol and lnc-NONH-SAT071210 shRNA co-treatment(P<0.01).Conclusion:Catalpol can inhibit expressions of inflammatory cytokines in serum and infiltration of lymphocyte in salivary gland of SS model mice,and inhibit salivary gland ductal fine epithelial inflammatory response and progression of SS by regulating lnc-NONHSAT071210.
