Role of Caspase-11/GSDMD non-classical pyroptosis pathway in ricin toxin-induced inflammation in RAW264.7 cells
10.3969/j.issn.1000-484X.2025.04.021
- VernacularTitle:Caspase-11/GSDMD非经典细胞焦亡途径在蓖麻毒素诱导RAW264.7细胞炎症反应中的作用
- Author:
Suli SONG
1
;
Mingxin DONG
;
Chengbiao SUN
;
Yan WANG
;
Ru LIN
;
Na XU
;
Wensen LIU
Author Information
1. 长春理工大学生命科学技术学院,长春 130012;中国农业科学院长春兽医研究所,长春 130122
- Publication Type:Journal Article
- Keywords:
Ricin toxin;
Pyroptosis;
Caspase-11;
GSDMD;
Inflammation
- From:
Chinese Journal of Immunology
2025;41(4):898-902,907
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore mechanism of Caspase-11/GSDMD non-classical cell pyroptosis pathway in inflammatory response of RAW264.7 monocyte macrophages induced by ricin toxin.Methods:Cell pyroptosis model induced by LPS+Nigericin was used as a positive control,and cells were induced with 40 ng/ml and 80 ng/ml of ricin toxin for 8 hours.Cell release of lactate dehydro-genase(LDH)was measured by LDH cell release assay;cell viability was assessed by flow cytometry(Annexin Ⅴ/PI double staining);gene expressions of Caspase-11,GSDMD,IL-1β and IL-18 were measured by RT-qPCR;protein levels of Caspase-11 and GSDMD were measured by Western blot;secretion levels of inflammatory cytokines IL-1β and IL-18 in cell supernatant were measured by ELISA.Results:Compared with normal control group,RAW264.7 cells treated with ricin toxin showed swelling,cell membrane rupture,significantly increased LDH release(P<0.001),significantly increased pyroptosis rate(P<0.001),significantly increased expression of pyroptosis-related genes Caspase-11,GSDMD,IL-1β and IL-18(P<0.05),significantly increased protein expressions of Caspase-11 and GSDMD(P<0.05),and significantly increased secretion levels of IL-1β and IL-18 in cell supernatant(P<0.05).Results of ricin toxin treatment group were consistent with LPS+Nigericin cell pyroptosis positive control group.Conclu-sion:Ricin toxin may induce cell pyroptosis in RAW264.7 cells through Caspase-11/GSDMD non-classical pathway,thereby promoting inflammatory response.
