Impacts of remifentanil on neuroinflammation in rats with cerebral infarction by regulating MIP-1α/CCR1 signaling pathway
10.3969/j.issn.1000-484X.2025.04.020
- VernacularTitle:瑞芬太尼调节MIP-1α/CCR1信号通路对脑梗死大鼠神经炎症的影响
- Author:
Jinliang XIAO
1
;
Weilian WANG
1
;
Jiapeng DAN
1
Author Information
1. 荆州市中心医院,长江大学附属荆州医院麻醉科,荆州 434000
- Publication Type:Journal Article
- Keywords:
Remifentanil;
MIP-1α/CCR1 signaling pathway;
Cerebral infarction;
Neuroinflammation
- From:
Chinese Journal of Immunology
2025;41(4):893-897
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore impacts of remifentanil on neuroinflammation in rats with cerebral infarction by regulating CC chemokine macrophage inflammatory protein-1α(MIP-1α)/CC chemokine receptor 1(CCR1)signaling pathway.Methods:Rats were randomly grouped into sham group,model group,remifentanil group,MIP-1α Ab group(MIP-1α neutralizing antibody,10 μg)and HY-U00350 group(CCR1 antagonist,100 μg),improved Longa suture method was applied to prepare a rat cerebral infarction model.Neural function scoring method was applied to evaluate neural function of rats;ELISA was applied to detect serum levels of TNF-α,IL-6 and IL-1β;HE staining was applied to observe pathological changes in rat hippocampal tissue,TTC staining method was applied to detect area of cerebral infarction in rats,TUNEL staining was applied to detect apoptosis rate of rat brain cells;RT-qPCR was applied to detect expressions of MIP-1α mRNA and CCR1 mRNA;Western blot was applied to detect expressions of MIP-1α and CCR1 proteins in rat brain tissue.Results:Compared with sham group,hippocampal neuron structure was seriously damaged in model group,neurological function score,levels of TNF-α,IL-6 and IL-1β,apoptosis rate,percentage of cerebral infarction area,expres-sions of MIP-1α,CCR1 gene and protein were obviously increased(P<0.05);compared with model group,morphological damage of neurons in remifentanil group,MIP-1α Ab group and HY-U00350 group was obviously reduced,neurological function score,levels of TNF-α,IL-6 and IL-1β,apoptosis rate,percentage of cerebral infarction area,expressions of MIP-1α,CCR1 gene and protein were obviously reduced(P<0.05);compared with remifentanil group,indicators of rats in MIP-1α Ab group had no statistical significance(P>0.05),levels of MIP-1α gene and protein obviously increased in HY-U00350 group,other indicators were also not statistically obvious(P>0.05).Conclusion:Remifentanil can reduce neuroinflammation in rats with cerebral infarction,which may be related to inhibition of MIP-1α/CCR1 signaling pathway.
