ABCA1-derived circRNA Regulates Cholesterol Homeostasis and Affects the Severity of Coronary Artery Lesions
10.3870/j.issn.1672-0741.25.01.011
- VernacularTitle:ABCA1来源的环状RNA调控胆固醇稳态影响冠脉病变程度
- Author:
Rongli WANG
1
;
Jing WANG
1
;
Hu DING
1
Author Information
1. 华中科技大学同济医学院附属同济医院心血管内科,武汉 430030
- Publication Type:Journal Article
- Keywords:
circRNA;
ABCA1;
cholesterol homeostasis;
coronary artery disease;
biomarker
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(2):143-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of circular RNA derived from the ABCA1 gene(circABCA1)in regulating choles-terol homeostasis and its clinical relevance to coronary artery diseases(CAD).Methods Real-time PCR and Western blot were used to assess the expression of ABCA1.Intracellular lipid deposition was detected by oil red O staining.The expression level of circABCA1 was detected in PBMCs collected from CAD patients.Spearman correlation analysis was used to explore the correla-tion between circABCA1 and lipid levels as well as the degree of coronary artery lesions.Multivariate logistic regression analysis was used to evaluate whether circABCA1 is an independent risk factor for coronary heart disease.Results ABCA1 expression was inhibited,total cholesterol levels and lipid deposition significantly elevated in cells after overexpression of circABCA1.In pa-tients with coronary artery disease,the expression level of circABCA1 was positively correlated with lipid levels(r=0.3545,P＜0.01)and Gensini score(r=0.3690,P＜0.01).The risk of coronary heart disease was increased after upregulation of circAB-CA1.Conclusion circABCA1 promotes intracellular lipid deposition by inhibiting ABCA1 expression and shows a significant positive correlation with the degree of coronary artery lesions,suggesting that circABCA1 is a functional RNA and a potential intervention target for the prevention and treatment of CAD.
