The effect of paeoniflorin on inflammatory response in rats with periapical periodontitis by regulating TLR4/MyD88/NF-κB signaling pathway
10.13431/j.cnki.immunol.j.20250003
- VernacularTitle:芍药苷调节TLR4/MyD88/NF-κB信号通路对根尖周炎大鼠炎症反应的影响
- Author:
Hui SHEN
1
;
Jing WANG
1
;
Yapeng ZHAO
1
;
Yaqiong CHEN
1
Author Information
1. 810000 西宁,青海红十字医院口腔科
- Publication Type:Journal Article
- Keywords:
Paeoniflorin;
TLR4/MyD88/NF-κB pathway;
Periapical periodontitis;
Inflammatory response
- From:
Immunological Journal
2025;41(1):24-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of paeoniflorin on the inflammatory response in rats with periapical periodontitis by regulating the TLR4/MyD88/NF-κB pathway.Methods Periapical periodontitis model was established in 72 rats,which were then randomly divided into 6 groups:model group,model+low-dose paeoniflorin(model+paeoniflorin-L,5 mg/kg)group,model+medium-dose paeoniflorin(model+paeoniflorin-M,10 mg/kg)group,model+high-dose paeoniflorin(model+paeoniflorin-H,20 mg/kg)group,and model+paeoniflorin-H+TLR4 activator(LPS)group(model+paeoniflorin-H+LPS,1 μg/kg LPS),with 12 rats in each group.In addition,12 normal rats were included as the control check(CK)group.The levels of CRP,IL-6 and IL-2 were detected by ELISA;the damage of periapical tissues was observed by HE staining;the number of tartrate-resistant acid phosphatase(TRAP)-positive osteoclasts was detected by TRAP staining;the level of cathepsin K and the proteins related to the TLR4/MyD88/NF-κB pathway were detected by Western blotting.Results There were very few inflammatory cells in the CK group,while there was a large accumulation of inflammatory cells in the model group.The expression levels of CRP,IL-6,IL-2,tissue protease K,osteoclast positive cells,TLR4,MyD88,p-NF-κB p65/NF-κB p65 proteins in the model group were higher than those in the CK group(P＜0.05).Compared with the model group,the above-mentioned indicators in the model+paeoniflorin-L group,model+paeoniflorin-M group,and model+paeoniflorin-H group decreased successively(P＜0.05).Compared with the model+paeoniflorin-H group,the above-mentioned indicators in the model+paeoniflorin-H+LPS group increased(P＜0.05).Conclusion Paeoniflorin may alleviate the inflammatory response in rats with periapical periodontitis by inhibiting the TLR4/MyD88/NF-κB signaling pathway.
