Expression of PLA2G2A and its significance in the vaginal wall of patients with pelvic organ prolapse
10.12007/j.issn.0258-4646.2025.11.014
- VernacularTitle:PLA2G2A在盆腔器官脱垂患者阴道壁中的表达及意义
- Author:
Jianfang GENG
1
;
Lei LI
1
;
Yajing SHI
1
;
Junnai WANG
1
;
Tingwei XIAO
1
;
Manman NAI
1
Author Information
1. 郑州大学第三附属医院盆底重建科,郑州 450052
- Publication Type:Journal Article
- Keywords:
pelvic organ prolapse;
vaginal wall;
fibroblasts;
PLA2G2A
- From:
Journal of China Medical University
2025;54(11):1042-1047
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of PLA2G2A and its significance in vaginal wall tissue of patients with pelvic organ pro-lapse(POP).Methods Twenty-three patients without POP(control group)and 26 patients with POP(POP group)admitted to the Third Affiliated Hospital of Zhengzhou University,between June 2023 and September 2024,were selected.Histological features were observed using hematoxylin and eosin and Masson's trichrome staining.Localization of PLA2G2A was detected using immunofluorescence.The PLA2G2A expression level was assessed using immunohistochemistry,real-time PCR,and Western blotting.After transfecting fibroblasts with silenced PLA2G2A,changes in collagen Ⅰ and collagen Ⅲ were measured.Results The histological structure of the vaginal wall in the POP group was significantly different from that in the control group.PLA2G2A was expressed in fibroblasts,with protein and mRNA expression levels higher than those in the control group(P＜0.05).After silencing PLA2G2A,collagen Ⅰ and collagen Ⅲ expression were upregulated.Conclusion High expression of PLA2G2A in vaginal wall tissue of patients with POP.Increased PLA2G2A expression may be closely related to the development and presence of POP.
