Therapeutic Effect of Chitosan-Collagen-Salvianolic Acid Slow-release Composite Membrane on Lumbar Spine and Epidural Scar Tissue After Lumbar Laminectomy in Rabbits
10.11969/j.issn.1673-548X.2025.02.010
- VernacularTitle:几丁糖-胶原-丹参素缓释复合膜对兔腰椎板切除术后腰椎及硬膜外瘢痕组织的治疗作用
- Author:
Ruiming ZENG
1
;
Jun KUANG
;
Xuan WAN
Author Information
1. 330006 南昌,江西中医药大学附属医院创伤骨科
- Publication Type:Journal Article
- Keywords:
Lumbar laminectomy;
Composite membrane material;
Sodium hyaluronate;
Hydroxyproline
- From:
Journal of Medical Research
2025;54(2):58-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the therapeutic effect of a chitosan-collagen-salvianolic acid slow-release composite mem-brane on lumbar spine and epidural scar tissue after lumbar laminectomy in rabbits.Methods Chitosan,type Ⅰ collagen,and salvianol-ic acid were used to prepare the composite membrane material in a specific ratio.After constructing a lumbar laminectomy animal model in New Zealand white rabbits,the material was implanted.The animals were sacrificed at 4 and 8 weeks after surgery,L1,L2 and T12 lumbar spine and epidural scar tissue were collected.Hematoxylin-eosin staining and Masson staining were used to detect pathological changes in lumbar spine bone tissue,and a biochemical kit was used to measure the hydroxyproline(HYP)content in scar tissue.Results At 4 and 8 weeks after surgery,the model group showed tight adhesion between the dura mater and scar tissue,with inability to separate blunt-ly.The model group had reduced trabecular number and thickness compared to the sham group,with trabecular fractures,disordered ar-rangement,disrupted collagen continuity,and increased HYP content.After implantation of different materials,the adhesion between the dura mater and scar tissue decreased,trabecular arrangement improved,trabecular number increased,bone collagen staining area signifi-cantly increased,collagen structure was restored,and HYP content at 4 weeks was significantly lower than the model group(P＜0.05).At 8 weeks,HYP content showed no significant difference compared to the model group,with only the model+CC group showed a signifi-cant decrease(P＜0.05).Conclusion The chitosan-collagen-salvianolic acid slow-release composite membrane promotes the heal-ing and repair of tissues after lumbar laminectomy in rabbits.
