Effect of melatonin in alleviating ovarian granulosa cell apoptosis induced by chronic hypoxia
10.12007/j.issn.0258-4646.2025.11.010
- VernacularTitle:褪黑素改善长期低氧诱导的卵巢颗粒细胞凋亡的分子机制
- Author:
Yinling XIU
1
;
Ying LIU
;
Kaixuan SUN
;
Panpan ZHAO
;
Shufang TANG
;
Jing ZHOU
;
Yitong ZHANG
;
Yuexin YU
Author Information
1. 中国人民解放军北部战区总医院生殖医学科,沈阳 110003
- Publication Type:Journal Article
- Keywords:
melatonin;
hypoxia;
rat;
ovary;
granulosa cell
- From:
Journal of China Medical University
2025;54(11):1017-1022
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of melatonin in alleviating hypoxia-induced apoptosis in ovarian gra-nulosa cells.Methods Rat ovarian granulosa cells were isolated and divided into normoxic,hypoxic,and melatonin groups.Hypoxia-induced injury models were established in the hypoxic and melatonin groups,and granulosa cells in the melatonin group were treated with melatonin.A total of 24 rats were randomized into the control,model,and intervention groups(n=8 per group).Rat models of declining ovarian function induced by long-term hypoxia were established in the model and intervention groups.The rats in the intervention group were intraperitoneally injected with melatonin.Cell proliferation was measured using a CCK-8 assay,and lactate secretion and HIF-1αprotein with a specific kit,respectively.The levels of estradiol and progesterone in the cell supernatant and rat serum were detected using ELISA.Granulosa cell apoptosis was detected by flow cytometry,ovarian morphology by HE staining,and Bax and caspase-3 expression by Western blotting.Results Compared with the normoxic group,the hypoxic group exhibited decreased granulosa cell proliferation,increased apoptosis,elevated lactate and HIF-1α levels,and reduced estradiol and progesterone levels(P＜0.05).Compared with hypoxic group,these changes were significantly reversed in the molatonin group(P＜0.05).Compared with the control group,the model group showed increased lactate,HIF-1α,Bax,and caspase-3 levels,decreased estradiol and progesterone levels,and reduced follicles.Compared with the model group,all the indicators were ameliorated in the intervention group(P＜0.05).Conclusion Melatonin alleviated hypoxia-induced granulosa cell apoptosis and promoted the recovery of ovarian function.
