Expression and clinical significance of TTK and MDM2 in cervical lesion tissue
10.12007/j.issn.0258-4646.2025.11.008
- VernacularTitle:TTK和MDM2在宫颈病变组织中的表达及临床意义
- Author:
Mengmeng FAN
1
;
Lili XIONG
;
Ruoqi DU
;
Doudou XU
;
Gailing FU
Author Information
1. 河南科技大学临床医学院,河南 洛阳 471000;河南科技大学第一附属医院妇科,河南 洛阳 471000
- Publication Type:Journal Article
- Keywords:
cervical lesion tissue;
serine/threonine and tyrosine kinase;
mouse dual microbody 2;
expression
- From:
Journal of China Medical University
2025;54(11):1005-1010
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and roles of serine/threonine and tyrosine kinases(TTK)and mouse dual microbody 2(MDM2)in cervical lesion tissue.Methods Patients(n=180)who underwent gynecological surgery at the First Affiliated Hospital of Henan University of Science and Technology from June 2022 to January 2024 were evaluated,including 50 patients in the cervical cancer group,50 patients in the high-grade lesion group,40 patients in the low-grade lesion group,and 40 patients in the non-cervical lesion(control)group.The mRNA expression and protein levels of TTK and MDM2 in each group were detected and compared by real-time quantitative PCR and immunohistochemistry,respectively.The diagnostic value of the mRNA expression levels of TTK and MDM2 in cer-vical cancer was analyzed using receiver operating characteristic curves.Results The mRNA levels of TTK and MDM2 significantly dif-fered among the four groups(P＜0.05);with increases in the lesion grade,the mRNA levels of TTK and MDM2 increased gradually and showed significant differences(P＜0.05).Immunohistochemical results showed that TTK was mainly located in the cytoplasm,whereas MDM2 was mainly located in the nucleus,with positive expression rates in cervical cancer tissues of 84.00％and 90.00％,respectively.These values were higher than those in other pathological tissues(P＜0.05).The mRNA levels of TTK and MDM2 were significantly cor-related with the degree of differentiation and lymph node metastasis(P＜0.05).The receiver operating characteristic curve showed that the areas under the curve of TTK and MDM2 mRNA for diagnosing cervical cancer were 0.803 and 0.798,respectively.Additionally,the area under the curve of the combined diagnosis was 0.912,which was higher than that observed for single detection(Z=2.230,2.553,P＜0.05).Conclusion The mRNA expression of TTK and MDM2 and their corresponding protein levels were increased in cervical lesions,demonstrating their potential as biological indicators for the clinical diagnosis of cervical cancer.
